Mouse IgG2a Isotype Control (20102) [Phycoerythrin]

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin a2/CD49b PE-conjugated Monoclonal Antibody (Catalog # FAB1233P, filled histogram) or Mouse IgG PE-conjugated Isotype Control Antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Ctrl
• Clone: 20102
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Mouse IgG2a Isotype Control (20102) [Phycoerythrin] Summary

• Immunogen: KLH
• Specificity: Selected for use as mouse IgG_2A isotype control for flow cytometry applications.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Control 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IgG2a Isotype Control (20102) [Phycoerythrin]

• IgG2A

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2a Isotype Control (IC003P)(35)

Publications using IC003P

• Jia, H;Moore, M;Wadhwa, M;Burns, C; Human iPSC-Derived Endothelial Cells Exhibit Reduced Immunogenicity in Comparison With Human Primary Endothelial Cells Stem cells international 2024-12-09 [PMID: 39687754] (Flow Cytometry Control, Human)
• Wang, B;Wang, T;Yang, C;Nan, Z;Ai, D;Wang, X;Wang, H;Qu, X;Wei, F; Co-inhibition of adenosine 2b receptor and programmed death-ligand 1 promotes the recruitment and cytotoxicity of natural killer cells in oral squamous cell carcinoma PeerJ 2023-08-30 [PMID: 37663280] (Flow Cytometry Control, Mouse)
• Hennequart, M;Pilley, SE;Labuschagne, CF;Coomes, J;Mervant, L;Driscoll, PC;Legrave, NM;Lee, Y;Kreuzaler, P;Macintyre, B;Panina, Y;Blagih, J;Stevenson, D;Strathdee, D;Schneider-Luftman, D;Gr�nroos, E;Cheung, EC;Yuneva, M;Swanton, C;Vousden, KH; ALDH1L2 regulation of formate, formyl-methionine, and ROS controls cancer cell migration and metastasis Cell reports 2023-05-26 [PMID: 37245210] (Flow Cytometry, Mouse)
• Koh, EK;Lee, HR;Son, WC;Park, GY;Bae, J;Park, YS; Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer Oncology letters 2023-06-01 [PMID: 37153058] (Flow Cytometry, Human)
• L Degenfeld-, S Gamperl, G Stefanzl, AK Schruef, I Sadovnik, K Bauer, D Smiljkovic, G Eisenwort, B Peter, G Greiner, E Hadzijusuf, J Schwaab, WR Sperr, G Hoermann, S Kopanja, Z Szépfalusi, K Hoetzeneck, P Jaksch, A Reiter, M Arock, P Valent Antineoplastic efficacy profiles of avapritinib and nintedanib in KIT D816V+ systemic mastocytosis: a preclinical study American journal of cancer research, 2023-02-15;13(2):355-378. 2023-02-15 [PMID: 36895976] (Flow Cytometry, Human)
• M Hassan, V Trung, D Bedi, S Shaddox, D Gunturu, C Yates, P Datta, T Samuel Interference with pathways activated by topoisomerase inhibition alters the surface expression of PD-L1 and MHC I in colon cancer cells Oncology Letters, 2022-12-09;25(1):41. 2022-12-09 [PMID: 36589674] (Flow Cytometry, Human)
• JT de Kay, J Carver, B Shevenell, AM Kosta, S Tsibulniko, E Certo, DB Sawyer, S Ryzhov, MP Robich Decreased expression of ErbB2 on left ventricular epicardial cells in patients with diabetes mellitus Cellular Signalling, 2022-05-21;96(0):110360. 2022-05-21 [PMID: 35609807] (Flow Cytometry, Human)
• S Ma, LS Mangala, W Hu, E Bayaktar, A Yokoi, W Hu, S Pradeep, S Lee, PD Piehowski, A Villar-Pra, SY Wu, MH McGuire, OD Lara, C Rodriguez-, CJ LaFargue, NB Jennings, KD Rodland, T Liu, V Kundra, PT Ram, S Ramakrishn, G Lopez-Bere, RL Coleman, AK Sood CD63-mediated cloaking of VEGF in small extracellular vesicles contributes to anti-VEGF therapy resistance Cell Reports, 2021-08-17;36(7):109549. 2021-08-17 [PMID: 34407412] (Isotype Control, Human)
• Johannes Nelke, Juliane Medler, Daniela Weisenberger, Andreas Beilhack, Harald Wajant CD40- and CD95-specific antibody single chain-Baff fusion proteins display BaffR-, TACI- and BCMA-restricted agonism mAbs 2020-08-26 [PMID: 32840410]
• Anne-Claire Lagrée, Fabienne Fasani, Clotilde Rouxel, Marine Pivet, Marie Pourcelot, Aurore Fablet et al. Bovine Organospecific Microvascular Endothelial Cell Lines as New and Relevant In Vitro Models to Study Viral Infections International Journal of Molecular Sciences 2020-07-24 [PMID: 32722052]
• Marloes van van Splunter, Olaf Perdijk, Henriëtte Fick-Brinkhof, Esther G. Floris-Vollenbroek, Ben Meijer, Sylvia Brugman et al. Plasmacytoid dendritic cell and myeloid dendritic cell function in ageing: A comparison between elderly and young adult women PLOS ONE 2019-12-12 [PMID: 31830086]
• N Malerba, P Benzoni, GM Squeo, R Milanesi, F Giannetti, LG Sadleir, G Poke, B Augello, AI Croce, A Barbuti, G Merla Generation of the induced human pluripotent stem cell lines CSSi009-A from a patient with a GNB5 pathogenic variant, and CSSi010-A from a CRISPR/Cas9 engineered GNB5 knock-out human cell line Stem Cell Res, 2019-08-22;40(0):101547. 2019-08-22 [PMID: 31479876] (Flow Cytometry, Human)
• Marloes van Splunter, Olaf Perdijk, Henriëtte Fick-Brinkhof, Anouk L. Feitsma, Esther G. Floris-Vollenbroek, Ben Meijer et al. Bovine Lactoferrin Enhances TLR7-Mediated Responses in Plasmacytoid Dendritic Cells in Elderly Women: Results From a Nutritional Intervention Study With Bovine Lactoferrin, GOS and Vitamin D Frontiers in Immunology 2018-11-20 [PMID: 30515164]
• B Ermon, CB Volpato, G Cattelan, R Silipigni, M Di Segni, C Cantaloni, M Casella, PP Pramstalle, G Pompilio, E Sommariva, V Meraviglia, A Rossini Derivation of human induced pluripotent stem cell line EURACi004-A from skin fibroblasts of a patient with Arrhythmogenic Cardiomyopathy carrying the heterozygous PKP2 mutation c.2569_3018del50 Stem Cell Res, 2018-09-06;32(0):78-82. 2018-09-06 [PMID: 30219716] (Isotype Control, Human)
• I De Pauw, F Lardon, J Van den Bo, H Baysal, E Fransen, V Deschoolme, P Pauwels, M Peeters, JB Vermorken, A Wouters Simultaneous targeting of EGFR, HER2 and HER4 by afatinib overcomes intrinsic and acquired cetuximab resistance in head and neck squamous cell carcinoma cell lines Mol Oncol, 2018-05-01;0(0):. 2018-05-01 [PMID: 29603584] (Flow Cytometry, Human)
• V Meraviglia, P Benzoni, S Landi, C Murano, M Langione, BM Motta, S Baratto, R Silipigni, M Di Segni, PP Pramstalle, D DiFrancesc, E Gazzerro, A Barbuti, A Rossini Generation of human induced pluripotent stem cells (EURACi001-A, EURACi002-A, EURACi003-A) from peripheral blood mononuclear cells of three patients carrying mutations in the CAV3 gene Stem Cell Res, 2017-12-18;27(0):25-29. 2017-12-18 [PMID: 29304398] (Flow Cytometry, Human)
• S Li, NL Sullivan, N Rouphael, T Yu, S Banton, MS Maddur, M McCausland, C Chiu, J Canniff, S Dubey, K Liu, V Tran, T Hagan, S Duraisingh, A Wieland, AK Mehta, JA Whitaker, S Subramania, DP Jones, A Sette, K Vora, A Weinberg, MJ Mulligan, HI Nakaya, M Levin, R Ahmed, B Pulendran Metabolic Phenotypes of Response to Vaccination in Humans Cell, 2017-05-11;169(5):862-877.e17. 5/11/2017 [PMID: 28502771]
• Y Wang, X Ying, H Xu, H Yan, X Li, H Tang The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals Int J Nanomedicine, 2017-02-17;12(0):1369-1384. 2017-02-17 [PMID: 28260885] (Isotype Control, Mouse)
• Sergey Ryzhov, Anton Matafonov, Cristi L. Galindo, Qinkun Zhang, Truc-Linh Tran, Daniel J. Lenihan et al. ERBB signaling attenuates proinflammatory activation of nonclassical monocytes American Journal of Physiology-Heart and Circulatory Physiology 2017-05-01 [PMID: 28235789] (Flow Cytometry Control, Human)
• Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand PLoS Pathog, 2016-12-01;12(12):e1006015. 2016-12-01 [PMID: 27907183] (Flow Cytometry, Human)
• Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure. PLoS ONE, 2015-08-04;10(8):e0134568. 2015-08-04 [PMID: 26241657] (Flow Cytometry, Human)
• Devevre E, Renovato-Martins M, Clement K, Sautes-Fridman C, Cremer I, Poitou C Profiling of the three circulating monocyte subpopulations in human obesity. J Immunol, 2015-03-18;194(8):3917-23. 2015-03-18 [PMID: 25786686] (Flow Cytometry, Human)
• Liu Y, Carson-Walter E, Walter K Targeting chemokine receptor CXCR7 inhibits glioma cell proliferation and mobility. Anticancer Res, 2015-01-01;35(1):53-64. 2015-01-01 [PMID: 25550535] (Flow Cytometry Control, Human)
• Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells. Stem Cells, 2015-01-01;33(1):133-45. 2015-01-01 [PMID: 25187207] (Flow Cytometry Control, Human)
• Liu Y, Carson-Walter E, Walter K Chemokine receptor CXCR7 is a functional receptor for CXCL12 in brain endothelial cells. PLoS ONE, 2014-08-01;9(8):e103938. 2014-08-01 [PMID: 25084358] (Flow Cytometry, Human)
• Peled A, Abraham M, Avivi I, Rowe J, Beider K, Wald H, Tiomkin L, Ribakovsky L, Riback Y, Ramati Y, Aviel S, Galun E, Shaw H, Eizenberg O, Hardan I, Shimoni A, Nagler A The high-affinity CXCR4 antagonist BKT140 is safe and induces a robust mobilization of human CD34+ cells in patients with multiple myeloma. Clin Cancer Res, 2013-11-18;20(2):469-79. 2013-11-18 [PMID: 24246358] (Flow Cytometry Control, Human)
• Jing Zhao, Yu-Chen Fan, Feng-Kai Sun, Ze-Hua Zhao, Li-Yuan Wang, Lei-Hua Hu et al. Peripheral Type I Interferon Receptor Correlated with Oxidative Stress in Chronic Hepatitis B Virus Infection Journal of Interferon & Cytokine Research 2013-08-01 [PMID: 23663046]
• Long , Kristin, Whitmore , Alan C, Ferris , Martin T, Sempowski , Gregory, McGee , Charles, Trollinger , Bianca, Gunn , Bronwyn, Heise , Mark T Dendritic cell immunoreceptor regulates Chikungunya virus pathogenesis in mice. J Virol, 2013-03-13;87(10):5697-706. 2013-03-13 [PMID: 23487448] (Flow Cytometry Control, Mouse)
• H Rauert, T Stühmer, R Bargou, H Wajant, D Siegmund TNFR1 and TNFR2 regulate the extrinsic apoptotic pathway in myeloma cells by multiple mechanisms Cell Death & Disease 2011-08-18 [PMID: 21850048]
• Taner SB, Pando MJ, Roberts A, Schellekens J, Marsh SG, Malmberg KJ, Parham P, Brodsky FM Interactions of NK cell receptor KIR3DL1*004 with chaperones and conformation-specific antibody reveal a functional folded state as well as predominant intracellular retention. J. Immunol., 2010-11-29;186(1):62-72. 2010-11-29 [PMID: 21115737] (Control, Human)
• McDermott DH, De Ravin SS, Jun HS Severe congenital neutropenia resulting from G6PC3 deficiency with increased neutrophil CXCR4 expression and myelokathexis. Blood, 2010-07-08;116(15):2793-802. 2010-07-08 [PMID: 20616219] (Control, Human)
• Patricia A Cronin, Jiang H Wang, H Paul Redmond Hypoxia increases the metastatic ability of breast cancer cells via upregulation of CXCR4 BMC Cancer 2010-12-01 [PMID: 20492653]
• Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes. J. Immunol., 2009-03-01;182(0):3112-20. 2009-03-01 [PMID: 19234208] (Control, Human)
• Ahern D, Lloyd CM, Robinson DS Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors. Allergy, 2009-02-07;64(8):1121-9. 2009-02-07 [PMID: 19208087] (Flow Cytometry, Human)
• Traves SL, Smith SJ, Barnes PJ, Donnelly LE Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2. J. Leukoc. Biol., 2004-05-20;76(2):441-50. 2004-05-20 [PMID: 15155777] (Flow Cytometry, Human)

FAQs for IgG2a Isotype Control (IC003P). (Showing 1 - 1 of 1 FAQs).

1. I would like to know the concentration of this antibody IC003P
   • This antibody is provided as 2.0ml at 0.025mg/ml.