Mouse IgG2a Isotype Control (20102) [Alexa Fluor® 488]

BG01V human embryonic stem cells were stained with Mouse Anti-Human Podocalyxin Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1658G, filled histogram) or Mouse IgG Alexa Fluor® 488-conjugated Isotype Control Antibody (Catalog # IC003G, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Ctrl
• Clone: 20102
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Mouse IgG2a Isotype Control (20102) [Alexa Fluor® 488] Summary

• Immunogen: KLH
• Specificity: Selected for use as mouse IgG 2A isotype control for flow cytometry applications.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Control 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IgG2a Isotype Control (20102) [Alexa Fluor® 488]

• IgG2A

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2a Isotype Control (IC003G)(16)

Publications using IC003G

• Sharma R, Earla B, Baidoo KE et al. Upregulation of Somatostatin Receptor Type 2 Improves 177Lu-DOTATATE Therapy in Receptor-Deficient Pancreatic Neuroendocrine Tumor Model Molecular cancer therapeutics 2023-07-22 [PMID: 37487000]
• Barnabas Irmer, Janes Efing, Lea Elisabeth Reitnauer, Allegra Angenendt, Saskia Heinrichs, Antonia Schubert et al. Extracellular vesicle-associated tyrosine kinase-like orphan receptors ROR1 and ROR2 promote breast cancer progression Cell Communication and Signaling 2023-07-10 [PMID: 37430307]
• Francisco Raúl RA贚 BORZONE, María Belén BEL蒒 GIORELLO, Leandro Marcelo MARCELO MARTINEZ, María Cecilia CECILIA SANMARTIN, LEONARDO FELDMAN, FEDERICO DIMASE et al. Senescent mesenchymal stem/stromal cells in pre-metastatic bone marrow of untreated advanced breast cancer patients Oncology Research 2023-05-24 [PMID: 37305388]
• K Chan, AG Farias, H Lee, F Guvenc, P Mero, KR Brown, H Ward, M Billmann, K Aulakh, A Astori, S Haider, E Marcon, U Braunschwe, S Pu, A Habsid, AH Yan Tong, N Christie-H, P Budylowski, A Ghalami, S Mubareka, F Maguire, A Banerjee, KL Mossman, J Greenblatt, SD Gray-Owen, B Raught, BJ Blencowe, M Taipale, C Myers, J Moffat Survival-based CRISPR genetic screens across a panel of permissive cell lines identify common and cell-specific SARS-CoV-2 host factors Heliyon, 2022-12-30;0(0):e12744. 2022-12-30 [PMID: 36597481] (Flow Cytometry Control, Human)
• J Matulien?, G Žvinys, V Petrauskas, A Kvietkausk, A Zakšauskas, K Shubin, A Zubrien?, L Baranauski, L Ka?enauska, S Kopanchuk, S Veiksina, V Paketuryt?, J Smirnovien, V Juozapaiti, A Mickevi?i?, V Michailovi, J Jachno, D Stravinski, A Sližien?, A Petroši?t?, HM Becker, J Kazokait?-, A Yaromina, E ?apkauskai, A Rinken, V Dudutien?, LJ Dubois, D Matulis Picomolar fluorescent probes for compound affinity determination to carbonic anhydrase IX expressed in live cancer cells Scientific Reports, 2022-10-21;12(1):17644. 2022-10-21 [PMID: 36271018] (Flow Cytometry, Human)
• E Bollinger, M Peloquin, J Libera, B Albuquerqu, E Pashos, A Shipstone, A Hadjipanay, Z Sun, G Xing, M Clasquin, JC Stansfield, B Tierney, S Gernhardt, CP Siddall, T Greizer, FJ Geoly, SR Vargas, LC Gao, G Williams, M Marshall, A Rosado, C Steppan, KJ Filipski, BB Zhang, RA Miller, RJ Roth Flach BDK inhibition acts as a catabolic switch to mimic fasting and improve metabolism in mice Molecular Metabolism, 2022-10-08;66(0):101611. 2022-10-08 [PMID: 36220546] (IHC Control, Mouse)
• T Lowin, C Kok, S Smutny, G Pongratz Impact of delta9-Tetrahydrocannabinol on Rheumatoid Arthritis Synovial Fibroblasts Alone and in Co-Culture with Peripheral Blood Mononuclear Cells Biomedicines, 2022-05-11;10(5):. 2022-05-11 [PMID: 35625855] (Flow Cytometry Control, Human)
• Claudia Huichalaf, Tyler L. Perfitt, Anna Kuperman, Renea Gooch, Ramesh C. Kovi, Karrie A. Brenneman et al. In vivo overexpression of frataxin causes toxicity mediated by iron-sulfur cluster deficiency Molecular Therapy - Methods & Clinical Development 2022-03-10 [PMID: 35252470]
• L El-Shennaw, AD Hoffmann, NK Dashzeveg, KM McAndrews, PJ Mehl, D Cornish, Z Yu, VL Tokars, V Nicolaescu, A Tomatsidou, C Mao, CJ Felicelli, CF Tsai, C Ostiguin, Y Jia, L Li, K Furlong, J Wysocki, X Luo, CF Ruivo, D Batlle, TJ Hope, Y Shen, YK Chae, H Zhang, VS LeBleu, T Shi, S Swaminatha, Y Luo, D Missiakas, GC Randall, AR Demonbreun, MG Ison, R Kalluri, D Fang, H Liu Circulating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2 Nature Communications, 2022-01-20;13(1):405. 2022-01-20 [PMID: 35058437] (Immunogold Electron Microscopy, Flow Cytometry, Human)
• Montes de Oca R, Alavi AS, Vitali N et al. Belantamab Mafodotin (GSK2857916) Drives Immunogenic Cell Death and Immune-mediated Antitumor Responses In Vivo Molecular Cancer Therapeutics 2021-10-01 [PMID: 34253590]
• Qiao Lu, Jia Liu, Shuai Zhao, Maria Florencia Gomez Castro, Maudry Laurent-Rolle, Jianbo Dong et al. SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2 Immunity 2021-06-01 [PMID: 34048708]
• DS Ahmet, HA Basheer, A Salem, D Lu, A Aghamohamm, P Weyerhäuse, A Bordiga, J Almeniawi, S Rashid, PA Cooper, SD Shnyder, V Vinader, K Afarinkia Application of small molecule FPR1 antagonists in the treatment of cancers Sci Rep, 2020-10-14;10(1):17249. 2020-10-14 [PMID: 33057069] (Flow Cytometry, Human)
• TT Ross, C Crowley, KL Kelly, A Rinaldi, DA Beebe, MP Lech, RV Martinez, S Carvajal-G, M Boucher, D Hirenallur, J Morin, AC Opsahl, SR Vargas, KK Bence, JA Pfefferkor, WP Esler Acetyl-CoA Carboxylase inhibition improves multiple dimensions of NASH pathogenesis in model systems Cell Mol Gastroenterol Hepatol, 2020-06-09;0(0):. 2020-06-09 [PMID: 32526482] (IHC, Rat)
• Jinfang Zhang, Ming Chen, Yasheng Zhu, Xiangpeng Dai, Fabin Dang, Junming Ren et al. SPOP Promotes Nanog Destruction to Suppress Stem Cell Traits and Prostate Cancer Progression Developmental Cell 2019-02-01 [PMID: 30595538]
• M Pedroza, RL Welschhans, SK Agarwal Targeting of cadherin-11 decreases skin fibrosis in the tight skin-1 mouse model PLoS ONE, 2017-11-07;12(11):e0187109. 2017-11-07 [PMID: 29112946] (IHC-P, Mouse)
• Michaela Bayerlová, Kerstin Menck, Florian Klemm, Alexander Wolff, Tobias Pukrop, Claudia Binder et al. Ror2 Signaling and Its Relevance in Breast Cancer Progression Frontiers in Oncology 2017-06-26 [PMID: 28695110]