![Immunohistochemistry-Paraffin: IgG Heavy Chain Antibody (RM116) - Azide and BSA Free [NBP3-25964] - Immunohistochemistry of Human Tonsil using Clone RM116.](http://images.novusbio.com/fullsize/nbp3-25964_rabbit-igg-heavy-chain-mab-rm116-azide-and-bsa-free-226202422321237.jpg "Immunohistochemistry-Paraffin: IgG Heavy Chain Antibody (RM116) - Azide and BSA Free [NBP3-25964] - Immunohistochemistry of Human Tonsil using Clone RM116.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, ICC/IF, IHC, ELISA |
| Clone | RM116 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Human IgG |
| Specificity | This antibody reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including gamma 1, gamma 2, gamma 3, and gamma 4. No cross reactivity with other human heavy chains, mouse IgG, rat IgG or goat IgG |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for IgG Heavy Chain Antibody (NBP3-25964)Find related products by research area.
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![Sandwich ELISA: IgG Heavy Chain Antibody (RM116) - Azide and BSA Free [NBP3-25964] - Sandwich ELISA using Clone RM116 as the capture antibody (100ng/well), and NBP3-18532 as the detection antibody (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.](http://images.novusbio.com/fullsize/nbp3-25964_rabbit-igg-heavy-chain-mab-rm116-azide-and-bsa-free-22620242311015.png "Sandwich ELISA: IgG Heavy Chain Antibody (RM116) - Azide and BSA Free [NBP3-25964] - Sandwich ELISA using Clone RM116 as the capture antibody (100ng/well), and NBP3-18532 as the detection antibody (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.")
![ELISA: IgG Heavy Chain Antibody (RM116) - Azide and BSA Free [NBP3-25964] - A titer ELISA using RM116. The plate was coated with different amounts of human IgG1. A serial dilution of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp3-25964_rabbit-igg-heavy-chain-mab-rm116-azide-and-bsa-free-22620242311090.png "ELISA: IgG Heavy Chain Antibody (RM116) - Azide and BSA Free [NBP3-25964] - A titer ELISA using RM116. The plate was coated with different amounts of human IgG1. A serial dilution of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
![ELISA: IgG Heavy Chain Antibody (RM116) - Azide and BSA Free [NBP3-25964] - ELISA of human immunoglobulins shows RM116 reacts to the gamma 1, gamma 2, gamma 3, gamma 4 heavy chains of human IgGs, and the Fc of human IgG. No cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp3-25964_rabbit-igg-heavy-chain-mab-rm116-azide-and-bsa-free-22620242232126.png "ELISA: IgG Heavy Chain Antibody (RM116) - Azide and BSA Free [NBP3-25964] - ELISA of human immunoglobulins shows RM116 reacts to the gamma 1, gamma 2, gamma 3, gamma 4 heavy chains of human IgGs, and the Fc of human IgG. No cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
![Sandwich ELISA: IgG Heavy Chain Antibody (RM116) - Azide and BSA Free [NBP3-25964] - Sandwich ELISA using Clone RM116 as the capture antibody (100ng/well), and NBP3-18532 as the detection antibody (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.](http://images.novusbio.com/fullsize/nbp3-25964_rabbit-igg-heavy-chain-mab-rm116-azide-and-bsa-free-226202422321236.png "Sandwich ELISA: IgG Heavy Chain Antibody (RM116) - Azide and BSA Free [NBP3-25964] - Sandwich ELISA using Clone RM116 as the capture antibody (100ng/well), and NBP3-18532 as the detection antibody (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")