Goat anti-Rabbit IgG (H+L) Secondary Antibody [FITC]

Images

 
Flow Cytometry: Goat anti-Rabbit, Mouse IgG (H+L) Secondary Antibody [FITC] [NB7159] - Both samples are cultured mouse microglial cells. The red is stained cells while the grey are cells with no primary antibody added ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
FITC
Concentration
1.0 mg/ml

Order Details

View Available Conjugates

Goat anti-Rabbit IgG (H+L) Secondary Antibody [FITC] Summary

Immunogen
This Goat anti-Rabbit IgG (H+L) Secondary Antibody [FITC] was developed against rabbit IgG-heavy and light chain.
Specificity
By immunoelectrophoresis and ELISA this Goat anti-Rabbit IgG (H+L) Secondary Antibody [FITC] reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:50 - 1:500
Reviewed Applications
Read 1 Review rated 5
using
NB7159 in the following applications:

Publications
Read Publication using NB7159.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS), 0.2%BSA
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7159)(1)

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
All Applications
Filter By Species
All Species

Review for IgG (H+L) Antibody (NB7159) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB7159:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry IgG (H+L) NB7159
Enlarge
5
reviewed by:
David Donley
Flow Mouse 07/17/2020
View

Summary

ApplicationFlow Cytometry
Sample TestedCell culture
SpeciesMouse
LotF24

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB7159) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Alkaline Phosphatase NB7157
Biotin NB7158
FITC NB7159
HRP NB7160
PerCP NB7156PCP

Additional IgG (H+L) Products

Array NB7159

Research Areas for IgG (H+L) Antibody (NB7159)

Find related products by research area.

Blogs on IgG (H+L)

There are no specific blogs for IgG (H+L), but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

David Donley
07/17/2020
Application: Flow
Species: Mouse