Goat anti-Rabbit IgG (H+L) Secondary Antibody [FITC]

Flow Cytometry: Goat anti-Rabbit, Mouse IgG (H+L) Secondary Antibody [FITC] [NB7159] - Both samples are cultured mouse microglial cells. The red is stained cells while the grey are cells with no primary antibody added (but the secondary antibody is present). Flow cytometry image submitted by a verified customer review.

• Reactivity: Rb
• Applications: Flow, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Goat
• Conjugate: FITC
• Concentration: 1.0 mg/ml

Goat anti-Rabbit IgG (H+L) Secondary Antibody [FITC] Summary

• Immunogen: This Goat anti-Rabbit IgG (H+L) Secondary Antibody [FITC] was developed against rabbit IgG-heavy and light chain.
• Specificity: By immunoelectrophoresis and ELISA this Goat anti-Rabbit IgG (H+L) Secondary Antibody [FITC] reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:50 - 1:500
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS), 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7159)(2)

Publications using NB7159

• Zwida KH, Kutzler MA. Canine splenic hemangiosarcoma cells express and activate luteinizing hormone receptors in vitro Am J Vet Res 2022-11-03 [PMID: 36327166] (ICC/IF, Rabbit)
  
  Details:
  Citation using the FITC version of this antibody.
• Lucarelli G, Galleggiante V, Rutigliano M et al. Metabolomic profile of glycolysis and the pentose phosphate pathway identifies the central role of glucose-6-phosphate dehydrogenase in clear cell-renal cell carcinoma. Oncotarget. [PMID: 25945836] (IHC-P)
  
  Details:
  Citation using the FITC form of this antibody.

Review for IgG (H+L) Antibody (NB7159) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 07/17/2020

Summary

• Application: Flow Cytometry
• Sample Tested: Cell culture
• Species: Mouse
• Lot: F24