Goat anti-Rabbit IgG (H+L) Secondary Antibody

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Flow Cytometry: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NB7156] - Both samples are cultured mouse microglial cells. The red is stained cells while the grey are cells with no primary antibody added (but the ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Goat anti-Rabbit IgG (H+L) Secondary Antibody Summary

Immunogen
This Goat anti-Rabbit IgG (H+L) Secondary Antibody was developed against rabbit IgG-heavy and light chain.
Specificity
By immunoelectrophoresis and ELISA this Goat anti-Rabbit IgG (H+L) Secondary Antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:30000
  • ELISA 1:1000-1:30000 (for coating plates use 1:100-1:500)
  • Immunocytochemistry/Immunofluorescence 1:200-1:2000
  • Immunohistochemistry 1:200-1:2000
  • Immunohistochemistry-Paraffin 1:200-1:2000
Application Notes
Use in Flow Cytometry was reported in the scientific literature (PMID: 30710055).
Publications
Read Publications using
NB7156 in the following applications:

  • 2 publications
  • 1 publication
  • IHC
    2 publications
  • 2 publications
  • WB
    13 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7156)(29)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 5 applications: ELISA, Flow, IHC, IHC-P, WB.


Filter By Application
ELISA
(2)
Flow
(1)
IHC
(2)
IHC-P
(2)
WB
(13)
All Applications
Filter By Species
Rat
(2)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB7156 Applications Species
Harb I, Ashour H, Sabry D et al. Nicorandil prevents the nephrotoxic effect of cyclosporine-A in albino rats through modulation of HIF-1alpha/VEGF /eNOS signaling J. Pharm. Pharmacol. Aug 21 2020 [PMID: 32822562]

Details:
Citation using the HRP format of this antibody.
Kaji S, Maki T, Ueda J et al. BCAS1-positive immature oligodendrocytes are affected by the alpha-synuclein-induced pathology of multiple system atrophy Acta Neuropathol Commun Jul 29 2020 [PMID: 32727582] (WB, Rat)

Details:
Citation using the HRP format of this antibody.
WB Rat
La Fuente JM, Sevilleja-Ortiz A, Garcia-Rojo E et al. Erectile dysfunction is associated with defective L-cysteine/hydrogen sulfide pathway in human corpus cavernosum and penile arteries Eur. J. Pharmacol. Jul 23 2020 [PMID: 32712093] (WB)

Details:
Citation using the HRP format of this antibody.
WB
Ho JJD, Balukoff NC, Theodoridis PR et al. A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism Nat Commun May 29 2020 [PMID: 32472050] (WB)

Details:
Citation using the HRP format of this antibody.
WB
Ismail A, Doghish AS, E M Elsadek B et al. Hydroxycitric acid potentiates the cytotoxic effect of tamoxifen in MCF-7 breast cancer cells through inhibition of ATP citrate lyase Steroids May 23 2020 [PMID: 32439410] (WB)

Details:
Citation using the Alkaline Phosphatase format of this antibody.
WB
AbuBakr N, Haggag T, Sabry D et al. Functional and histological evaluation of bone marrow stem cell-derived exosomes therapy on the submandibular salivary gland of diabetic Albino rats through TGF beta Smad3 signaling pathway Heliyon May 9 2020 [PMID: 32382678] (WB) WB
Choi JW, Lee SK, Kim MJ et al. Piperine ameliorates the severity of fibrosis via inhibition of TGF-beta SMAD signaling in a mouse model of chronic pancreatitis Mol Med Rep Sep 6 2019 [PMID: 31485676] (WB) WB
Mehta N, Maddineni S, Mathews II et al. Structure and Functional Binding Epitope of V-domain Ig Suppressor of T Cell Activation Cell Rep. 2019 Sep 3 [PMID: 31484064] (ELISA)

Details:
Citation used the HRP format of this antibody.
ELISA
Gunasekar P, Satish M, Dabestani P et al. Modulation of Cathepsin L Expression in the Coronary Arteries of Atherosclerotic Swine J Surg Res Aug 5 2019 [PMID: 31377485] (WB) WB
Grillo SL, McDevitt DS, Voas MG et al. Adenosine receptor expression in the adult zebrafish retina Purinergic Signal Jul 6 2019 [PMID: 31273575] (WB) WB
Show All 29 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB7156) (0)

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Other Available Formats

Alkaline Phosphatase NB7157
Biotin NB7158
FITC NB7159
HRP NB7160
PerCP NB7156PCP

Additional IgG (H+L) Products

Array NB7156

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