Donkey anti-Mouse IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed)

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Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB120-7061] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase donkey secondary antibody ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP
Format
Pre-absorbed
Concentration
1.0 mg/ml

Donkey anti-Mouse IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed) Summary

Immunogen
Full length native mouse IgG (purified).
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:20000
  • ELISA 1:180000
  • Immunohistochemistry 1:1000 - 1:5000
Readout System
Publications
Read Publications using
NB120-7061 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml BSA (Immunoglobulin and Protease free). DO NOT add Sodium Azide!
Preservative
0.01% Gentamicin Sulfate
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB120-7061)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ELISA, WB.


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ELISA
(1)
WB
(1)
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(1)
Mouse
(1)
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