Donkey anti-Mouse IgG (H+L) Secondary Antibody [DyLight 594]


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Product Details

Reactivity MuSpecies Glossary
Applications WB, ELISA, FLISA, IHC, IHC-Fr, IHC-P
DyLight 594

Order Details

Donkey anti-Mouse IgG (H+L) Secondary Antibody [DyLight 594] Summary

This Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against mouse IgG whole molecule.
Assay of Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey serum, Mouse IgG and Mouse serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep serum proteins. Specificity was confirmed using ELISA at less than 1.0% cross reactivity against IgG proteins purified from the species listed above. This antibody was pre-adsorbed against bovine chicken goat guinea pig hamster horse human rabbit rat & sheep serum proteins.
Immunogen affinity purified


  • Western Blot : 1:2000 - 1:10000
  • ELISA : 1:20000 - 1:100000
  • Immunohistochemistry : 1:1000 - 1:5000
  • Immunohistochemistry-Frozen : 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin : 1:1000 - 1:5000
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Immunogen affinity purified


DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.


Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).


1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Other Available Formats

Alkaline Phosphatase NB120-7062
Allophycocyanin/Cy7 NB120-7056APCCY7
Biotin NB120-7060
DyLight 405 NBP1-72941
DyLight 488 NBP1-72931
DyLight 549 NBP1-72933
DyLight 550 NB120-7056R
DyLight 594 NB120-7056DL594
DyLight 649 NBP1-72935
DyLight 650 NB120-7056C
DyLight 680 NBP1-72937
DyLight 755 NB120-7056IR
DyLight 800 NBP1-72939
FITC NB120-7057
HRP NB120-7061
Janelia Fluor 549 NB120-7056JF549
Janelia Fluor 646 NB120-7056JF646
PE/Atto594 NB120-7056PEATT594
PE/Cy5.5 NB120-7056PECY55
PE/Cy7 NB120-7056PECY7
Rhodamine NB120-7058
Texas Red NBP1-73647

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Array NB120-7056DL594

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