Donkey anti-Mouse IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed)

Images

 
Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-absorbed) [NB120-7056] - Rabbit anti-Transferrin, Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST. Detection shown was using DyLight549 Donkey ...read more
Western Blot: Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-absorbed) [NB120-7056] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Biotin donkey secondary ...read more
Fluorophore-linked immunosorbent assay: Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-absorbed) [NB120-7056] - Fluorophore-linked immunosorbent assay: Donkey anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, FLISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Donkey
Conjugate
Unconjugated
Format
Pre-adsorbed
Concentration
1 mg/ml

Donkey anti-Mouse IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed) Summary

Immunogen
Mouse IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Frozen 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
Application Notes
Suitable for immunoblotting (western or dot blot), ELISA, electron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
Publications
Read Publications using
NB120-7056 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB120-7056)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ELISA, WB.


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(1)
WB
(1)
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(1)
Mouse
(1)
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