Donkey anti-Mouse IgG (H+L) Secondary Antibody [PE/Cy5.5]

Images

 
There are currently no images for IgG (H+L) Antibody (NB120-7056PECY55).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, FLISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Donkey
Conjugate
PE/Cy5.5

Order Details

Donkey anti-Mouse IgG (H+L) Secondary Antibody [PE/Cy5.5] Summary

Immunogen
This Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against mouse IgG whole molecule.
Specificity
Assay of Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey serum, Mouse IgG and Mouse serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep serum proteins. Specificity was confirmed using ELISA at less than 1.0% cross reactivity against IgG proteins purified from the species listed above. This antibody was pre-adsorbed against bovine chicken goat guinea pig hamster horse human rabbit rat & sheep serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB120-7056PECY55) (0)

There are no publications for IgG (H+L) Antibody (NB120-7056PECY55).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IgG (H+L) Antibody (NB120-7056PECY55) (0)

There are no reviews for IgG (H+L) Antibody (NB120-7056PECY55). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IgG (H+L) Antibody (NB120-7056PECY55) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Alkaline Phosphatase NB120-7062
Allophycocyanin/Cy7 NB120-7056APCCY7
Biotin NB120-7060
DyLight 405 NBP1-72941
DyLight 488 NBP1-72931
DyLight 549 NBP1-72933
DyLight 550 NB120-7056R
DyLight 594 NB120-7056DL594
DyLight 649 NBP1-72935
DyLight 650 NB120-7056C
DyLight 680 NBP1-72937
DyLight 755 NB120-7056IR
DyLight 800 NBP1-72939
FITC NB120-7057
HRP NB120-7061
Janelia Fluor 549 NB120-7056JF549
Janelia Fluor 646 NB120-7056JF646
PE/Atto594 NB120-7056PEATT594
PE/Cy5.5 NB120-7056PECY55
PE/Cy7 NB120-7056PECY7
Rhodamine NB120-7058
Texas Red NBP1-73647

Additional IgG (H+L) Products

Array NB120-7056PECY55

Research Areas for IgG (H+L) Antibody (NB120-7056PECY55)

Find related products by research area.

Blogs on IgG (H+L)

There are no specific blogs for IgG (H+L), but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Donkey anti-Mouse IgG (H+L) Secondary Antibody [PE/Cy5.5] and receive a gift card or discount.