Rabbit anti-Human IgG (H L) Secondary Antibody [Biotin]

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Western Blot: Rabbit anti-Human IgG (H+L) Secondary Antibody [Biotin] [NB120-6758] - Lane 1: Human IgG Load: 50 ng per lane Primary antibody: Human IgG (H&L) Antibody Biotin Conjugated at 1:1000 for 60 min RT Secondary ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

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Rabbit anti-Human IgG (H+L) Secondary Antibody [Biotin] Summary

Immunogen
human IgG whole molecule
Localization
Secreted
Specificity
Human IgG (H and L)
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • ELISA 1:20000-1:100000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
Application Notes
As well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Peptide ELISA was reported in scientific literature.
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Publications
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NB120-6758 in the following applications:

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Human IgG Antibody (NB120-6758)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: PEP-ELISA.


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PEP-ELISA
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