Human lambda light chain Antibody (2496A) [Unconjugated]

Western blot shows lysates of human kappa light chain purified protein (negative sample), human lambda light chain purified protein, human serum, primate serum, mouse serum (negative sample), and rat serum (negative sample). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Primate Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB100492) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Ig Lambda Light Chain at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Pm
• Applications: WB
• Clone: 2496A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Human lambda light chain Antibody (2496A) [Unconjugated] Summary

• Immunogen: Human Ig Lamda Light Chain
• Specificity: Detects human Ig Lambda Light Chain in direct ELISAs. Detects human and primate Ig Lambda Light Chain in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human lambda light chain Antibody (2496A) [Unconjugated]

• Bence Jones protein
• BJP
• Constant region of lambda light chains
• HCAK 1
• HCAK1
• Ig kappa chain C region
• Ig lambda chain C regions
• Ig Lambda Light Chain
• IGKC
• IGLC 1
• IGLC 2
• IGLC 3
• IGLC
• IGLC1
• IGLC2
• IGLC3
• Immunoglobulin kappa constant region
• Immunoglobulin kappa constant
• Immunoglobulin kappa light chain
• Immunoglobulin lambda constant 1
• Kappa 1 immunoglobulin light chain
• Km
• Mcg marker
• MGC111575
• MGC62011
• MGC72072
• MGC88770
• MGC88771
• MGC88809
• Paraprotein

Background

The immunoglobulin light chain is the smaller subunit of an antibody and in humans can be expressed in two types: as a kappa ( kappa ) or a lambda ( lambda ) chain.  Antibodies are produced by B-Cells that are clonal and each expresses only one type of light chain. The light chain class remains fixed for the life of the B lymphocyte. The ratio of kappa and lambda light chains can be used to determine disease status by immunohistochemistry or ELISA based assays.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.