Human lambda light chain Antibody (1000011) [Unconjugated]

IgLambda was detected in immersion fixed paraffin-embedded sections of human Tonsil using Mouse Anti-Human IgLambda, Monoclonal Antibody (Catalog # MAB10049) at 0.5ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

Western blot shows human kappa light chain protein, human lambda light chain protein, human serum, primate serum, mouse serum, and rat serum. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB10049) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ig Lambda Light Chain at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human kappa light chain protein (negative control), human lambda light chain protein, and human serum, loaded at 0.2 mg/mL. A specific band was detected for Ig Lambda Light Chain at approximately 35 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB10049) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Human Ig Lambda Light Chain protein was serially diluted 2-fold and captured by Mouse Anti-Human Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB100491) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB10049) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Ig Lambda Light Chain was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human Ig Lambda Light Chain Monoclonal Antibody (Catalog # MAB10049) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, Simple Western, ELISA, IHC, mIF
• Clone: 1000011
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Human lambda light chain Antibody (1000011) [Unconjugated] Summary

• Immunogen: Ig Lambda Light Chain
• Specificity: Detects Human Ig Lambda Light Chain in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 3-25 ug/mL
  • Multiplex Immunofluorescence 0.5 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human lambda light chain Antibody (1000011) [Unconjugated]

• Bence Jones protein
• BJP
• Constant region of lambda light chains
• HCAK 1
• HCAK1
• Ig kappa chain C region
• Ig lambda chain C regions
• Ig Lambda Light Chain
• IGKC
• IGLC 1
• IGLC 2
• IGLC 3
• IGLC
• IGLC1
• IGLC2
• IGLC3
• Immunoglobulin kappa constant region
• Immunoglobulin kappa constant
• Immunoglobulin kappa light chain
• Immunoglobulin lambda constant 1
• Kappa 1 immunoglobulin light chain
• Km
• Mcg marker
• MGC111575
• MGC62011
• MGC72072
• MGC88770
• MGC88771
• MGC88809
• Paraprotein

Background

The immunoglobulin light chain is the smaller subunit of an antibody and in humans can be expressed in two types: as a kappa ( kappa ) or a lambda ( lambda ) chain.  Antibodies are produced by B-Cells that are clonal and each expresses only one type of light chain. The light chain class remains fixed for the life of the B lymphocyte. The ratio of kappa and lambda light chains can be used to determine disease status by immunohistochemistry or ELISA based assays.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.