![ELISA: Human Coronavirus Spike S1 Antibody - (HCoV-OC43) [NBP3-14863] - Antigen-down ELISA with HCoV-229E, HCoV-HKU1, HCoV-OC43, HCoV-NL63, SARS-CoV and SARS-CoV-2 spike subunit 1 proteins coated at 0.5ug. Primary antibody dilutions (1000 ng/ml to 0.24 ng/ml) added and 100ul of goat anti rabbit IgG HRP secondary antibody (1:10,000). Detection carried out using TMB substrate (15 min.), reaction stopped and absorbance read at 405/450nm.](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-S1-Antibody-HCoV-OC43-ELISA-NBP3-14863-img0001.jpg "ELISA: Human Coronavirus Spike S1 Antibody - (HCoV-OC43) [NBP3-14863] - Antigen-down ELISA with HCoV-229E, HCoV-HKU1, HCoV-OC43, HCoV-NL63, SARS-CoV and SARS-CoV-2 spike subunit 1 proteins coated at 0.5ug. Primary antibody dilutions (1000 ng/ml to 0.24 ng/ml) added and 100ul of goat anti rabbit IgG HRP secondary antibody (1:10,000). Detection carried out using TMB substrate (15 min.), reaction stopped and absorbance read at 405/450nm.")
| Reactivity | VSpecies Glossary |
| Applications | ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant HCoV-OC43 spike subunit 1 antigen |
| Specificity | Polyclonal antibody against human coronavirus OC43 (HCoV-OC43) spike subunit 1 (S1) protein. It shows some cross-reactivity towards other HCoV spike subunit 1 (S1) proteins and SARS-CoV S1 in ELISA, but not to SARS-CoV-2 S1 protein. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 0.2 um filtered. |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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