HSV VP16 Antibody

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Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
5.8 mg/ml

Order Details

HSV VP16 Antibody Summary

Immunogen
Synthetic peptide corresponding to amino acids 474-487 of the herpes simplex virus HSV VP16 protein, conjugated to KLH via an N-terminal added cysteine.
Specificity
Recognizes HSV VP16 fusion protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunoprecipitation 10ug

Reactivity Notes

Tag specific

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
5.8 mg/ml
Purity
Immunogen affinity purified

Background

VP16 from herpes simple virus (HSV) is a strong transcriptional activator of immediate early viral genes.1 Due to its lack of DNA binding activity, VP16 is recruited to DNA via interaction with DNA binding proteins.2 Ptashne et al. showed that VP16 can function as a potent transcriptional activator in mammalian cells when fused to the DNA binding domain of the yeast transcriptional activator GAL4.3 These properties were taken into consideration in the development of a mammalian version of the Yeast Two-Hybrid System.4-6 The Yeast Two-Hybrid System is a powerful method for characterizing and screening for protein-protein interactions. In the original version of the system, GAL4 DNA binding domain is fused to protein X, and GAL4 activation domain is fused to protein Y. Neither hybrid is capable of activating transcription individually. Upon interaction between proteins X and Y, the DNA binding and activator domains are brought in close proximity. As a result, the transcriptional activity of GAL4 is reconstituted at the promoter, and monitored with a reporter gene.4 Additional two-hybrid systems were developed using different DNA binding and activation domains as well as different reporter genes.5-9 The VP16 activator is used in yeast and mammalian systems. In yeast, its DNA interaction partner is typically LexA.10 In mammalian systems, interaction between two proteins is detected through recruitment of VP16 and GAL4 DNA binding domain to GAL4 operators present in the reporter gene.7,9-11

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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