![Western Blot: HRP Antibody [NB600-894] - Western Blot Results of Rabbit HRP antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: HRP (HRP). Primary Antibody: Anti-HRP at 1:100in 0.75%Casein/TBST overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG mx10 DyLight (TM) 649 at 1:5000 in blocking buffer Buffer for 30 mins at RT. Block: 0.75%Casein/TBST for 30 min at RT. Expected MW: ~44kDa.](http://images.novusbio.com/fullsize/HRP-Antibody-Western-Blot-NB600-894-img0001.jpg "Western Blot: HRP Antibody [NB600-894] - Western Blot Results of Rabbit HRP antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: HRP (HRP). Primary Antibody: Anti-HRP at 1:100in 0.75%Casein/TBST overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG mx10 DyLight (TM) 649 at 1:5000 in blocking buffer Buffer for 30 mins at RT. Block: 0.75%Casein/TBST for 30 min at RT. Expected MW: ~44kDa.")
| Reactivity | AllSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | LYOPH |
| Description | This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Peroxidase [Horseradish] Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | HRP [Horseradish] |
| Localization | Secreted |
| Specificity | Cross reactivity against HRP from other tissues and species may occur but have not been specifically determined. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Multi-step |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This product has been tested by western blotting. Specific conditions for reactivity should be optimized by the end user. |
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| Storage | Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent) |
| Publication using NB600-894 | Applications | Species |
|---|---|---|
| Lin X, Wang L, Wang F Chondromodulin-I suppresses tumorigenesis of human osteosarcoma cells Mol Med Rep 2017-01-01 [PMID: 28983591] |
Secondary Antibodies |
Isotype Controls |
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Ultrasensitive IHC Detection with HRP-Polymer Conjugates Signal amplification methods are widely used in immunohistochemistry (IHC) for detection of rare epitopes and low abundance antigens. While many of these techniques such as the avidin-biotin complex (ABC) method improve staining, they frequently re... Read full blog post. |
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5 Stars for the Lightning-Link HRP Antibody Labeling Kit Novus' Lightning-Link HRP Antibody Labeling Kit (cat# 701-0000) has received some glowing product reviews and customer feedback lately. One satisfied customer wrote, "Fast, efficient, stable direct HRP conjugation - best reagent we have found..." Anot... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")