![Western Blot: HPV18 E7 Antibody (HL2522) - Azide and BSA Free [NBP3-25512] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 18 E7 antibody [HL2522] (NBP3-25512) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25512_rabbit-hpv18-e7-mab-hl2522-azide-and-bsa-free-2120249403828.jpg "Western Blot: HPV18 E7 Antibody (HL2522) - Azide and BSA Free [NBP3-25512] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 18 E7 antibody [HL2522] (NBP3-25512) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ELISA, LFA |
| Clone | HL2522 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Rabbit HPV18 E7 Antibody (HL2522) - Azide and BSA Free (NBP3-25512) is a recombinant monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Full length HPV18 E7 |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![ELISA: HPV18 E7 Antibody (HL2522) - Azide and BSA Free [NBP3-25512] - Sandwich ELISA detection of recombinant Human Papillomavirus type 18 E7 protein, His tag using antibodies as below. Capture: Human Papillomavirus type 18 E7 antibody [HL2522] (NBP3-25512) (5 ug/mL) Detection: Human Papillomavirus type 18 E7 antibody [GT881] (NBP3-13610) (1 ug/mL)](http://images.novusbio.com/fullsize/nbp3-25512_rabbit-hpv18-e7-mab-hl2522-azide-and-bsa-free-2120249393533.jpg "ELISA: HPV18 E7 Antibody (HL2522) - Azide and BSA Free [NBP3-25512] - Sandwich ELISA detection of recombinant Human Papillomavirus type 18 E7 protein, His tag using antibodies as below. Capture: Human Papillomavirus type 18 E7 antibody [HL2522] (NBP3-25512) (5 ug/mL) Detection: Human Papillomavirus type 18 E7 antibody [GT881] (NBP3-13610) (1 ug/mL)")
![ELISA: HPV18 E7 Antibody (HL2522) - Azide and BSA Free [NBP3-25512] - Sandwich ELISA detection of recombinant Human Papillomavirus type 18 E7 protein, His tag using antibodies as below. Capture: Human Papillomavirus type 18 E7 antibody [HL2522] (NBP3-13610) (5 ug/mL) Detection: Human Papillomavirus type 18 E7 antibody [HL2522] (NBP3-25512) (1 ug/mL)](http://images.novusbio.com/fullsize/nbp3-25512_rabbit-hpv18-e7-mab-hl2522-azide-and-bsa-free-2120249415933.jpg "ELISA: HPV18 E7 Antibody (HL2522) - Azide and BSA Free [NBP3-25512] - Sandwich ELISA detection of recombinant Human Papillomavirus type 18 E7 protein, His tag using antibodies as below. Capture: Human Papillomavirus type 18 E7 antibody [HL2522] (NBP3-13610) (5 ug/mL) Detection: Human Papillomavirus type 18 E7 antibody [HL2522] (NBP3-25512) (1 ug/mL)")
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HPV18 E7 antibody [HL2522] (NBP3-25512) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25512_rabbit-hpv18-e7-mab-hl2522-azide-and-bsa-free-western-blot-1411202518294111.jpg "Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HPV18 E7 antibody [HL2522] (NBP3-25512) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Detection of HPV18 E7 protein by lateral flow assay using the monoclonal antibody pair. Capture: HPV18 E7 antibody [HL2522] (NBP3-25512) Detection: HPV18 E7 antibody [GT881]](http://images.novusbio.com/fullsize/nbp3-25512_rabbit-hpv18-e7-mab-hl2522-azide-and-bsa-free-lateral-flow-assay-141120251839105.jpg "Detection of HPV18 E7 protein by lateral flow assay using the monoclonal antibody pair. Capture: HPV18 E7 antibody [HL2522] (NBP3-25512) Detection: HPV18 E7 antibody [GT881]")
![Detection of HPV18 E7 protein by lateral flow assay using the indicated recombinant monoclonal antibodies. GT881 : HPV18 E7 antibody [GT881] : Recombinant full-length Papillomavirus type 18 E7 protein](http://images.novusbio.com/fullsize/nbp3-25512_rabbit-hpv18-e7-mab-hl2522-azide-and-bsa-free-lateral-flow-assay-1411202518394112.jpg "Detection of HPV18 E7 protein by lateral flow assay using the indicated recombinant monoclonal antibodies. GT881 : HPV18 E7 antibody [GT881] : Recombinant full-length Papillomavirus type 18 E7 protein")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")