HPV16 E7 Antibody (HL1821) - Azide and BSA Free

Western Blot: HPV16 E7 Antibody (HL1821) - Azide and BSA Free [NBP3-25511] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (NBP3-25511) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

ELISA: HPV16 E7 Antibody (HL1821) - Azide and BSA Free [NBP3-25511] - Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag using antibodies as below. Capture: Human Papillomavirus type 16 E7 antibody [HL1821] (NBP3-25511) (5 ug/mL) Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1647] (NBP3-25510) (1 ug/mL) Please notice that NBP3-25510 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25511. Please contact us for custom HRP-conjugated antibody.

Western Blot: HPV16 E7 Antibody (HL1821) - Azide and BSA Free [NBP3-25511] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (NBP3-25511) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

ELISA: HPV16 E7 Antibody (HL1821) - Azide and BSA Free [NBP3-25511] - Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag using antibodies as below. Capture: Human Papillomavirus type 16 E7 antibody [HL1647] (NBP3-25510) (5 ug/mL) Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1821] (NBP3-25511) (1 ug/mL) Please notice that NBP3-25511 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25510. Please contact us for custom HRP-conjugated antibody.

Western Blot: HPV16 E7 Antibody (HL1821) - Azide and BSA Free [NBP3-25511] - Human papillomavirus protein (100 ng) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (NBP3-25511) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Lateral Flow Assay: HPV16 E7 Antibody (HL1821) - Azide and BSA Free [NBP3-25511] - Detection of Human Papillomavirus type 16 E7 protein by lateral flow assay using the monoclonal antibody pair. Capture: Human Papillomavirus type 16 E7 antibody (NBP3-25510 [HL1647]) Detection: Human Papillomavirus type 16 E7 antibody (NBP3-25511 [HL1821]) Samples (10 ng) : 1. HPV16 E7 protein 2. HPV18 E7 protein 3. Lysis buffer

• Reactivity: V-Vi
• Applications: WB, ELISA, LFA
• Clone: HL1821
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HPV16 E7 Antibody (HL1821) - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Full length HPV16 E7 recombinant protein
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA Assay dependent
  • Lateral Flow Assay Assay dependent
  • Sandwich ELISA Assay dependent
  • Western Blot Assay dependent

Reactivity Notes

Human Papillomavirus type 16

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for HPV16 E7 Antibody (HL1821) - Azide and BSA Free

• E7
• HPV16 E7
• HpV16gp2
• Links Transforming protein human papillomavirus 16
• Protein E7
• Transforming protein human papillomavirus 16
• Transforming protein

Background

The HPV E7 proteins are small (HPV16 E7 comprising 98 amino acids, 15kDa), zinc binding phosphoproteins which are localised in the nucleus. They are structurally and functionally similar to the E1A protein of subgenus C adenoviruses. The first 16 amino-terminal amino acids of HPV16 E7 contain a region homologous to a segment of the conserved region 1 (CR1) of the E1A protein of subgenus C adenoviruses. The next domain, up to amino acid 37, is homologous to the entire region 2 (CR2) of E1A. Genetic studies have established that these domains are required for cell transformation in vitro, suggesting similarities in the mechanism of transformation by these viruses. The CR2 homology region contains the LXCXE motif (residues 22-26) involved in binding to the tumour suppressor protein pRb. This sequence is also present in SV40 and polyoma large T antigens. The high risk HPV E7 proteins (of, for example, types 16 and 18) have an approximately ten-fold higher affinity for pRb protein than the low risk HPV E7 proteins (of, for example, type 6). Association of the E7 protein with pRb promotes cell proliferation by the same mechanism as the E1A proteins of adenoviruses and SV40 large T antigen. Recent studies have shown that E7 promotes degradation of Rb family proteins rather than simply inhibiting their function by complex formation. The CR2 region also contains the casein kinase II phosphorylation site (residues 31 and 32). Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.