HPV16 E1+E4 Antibody (TVG 402)

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Product Details

Summary
Reactivity ViSpecies Glossary
Applications WB, ELISA, IHC, IP
Clone
TVG 402
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

HPV16 E1+E4 Antibody (TVG 402) Summary

Immunogen
Antigen for hybridoma production was expressed as a b galactosidase fusion protein using the pEX expression system and was consequently cleaved to release the E1/E4 polypeptide.
Epitope
Amino acids 36-41.
Localization
Nuclear
Specificity
The epitope recognized by this antibody survived brief fixation in 5% formaldehyde, but not prolonged fixation.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Theoretical MW
10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human papilloma virus

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for HPV16 E1+E4 Antibody (TVG 402)

  • ATP dependent helicase E1
  • E1
  • E4
  • HPV 16 ATP dependent helicase E1
  • HPV 16 E1
  • HPV 16 E4
  • HPV 16 replication protein E1
  • HPV16 ATP dependent helicase E1
  • HPV16 E1
  • HPV16 E4
  • HPV16 replication protein E1
  • Human papilloma virus type 16 ATP dependent helicase E1
  • Human papilloma virus type 16 E1
  • Human papilloma virus type 16 E4
  • Human papilloma virus type 16 replication protein E1
  • Human papilloma virus type 16
  • Human papillomavirus type 16 ATP dependent helicase E1
  • Human papillomavirus type 16 E1
  • Human papillomavirus type 16 E4
  • Human papillomavirus type 16 replication protein E1
  • Human papillomavirus type 16
  • Replication protein E1
  • Replication protein

Background

HPV16 is an ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. HPV16, in comparison to HPV types 6 and 11, is more often associated with malignant genital cancers in humans. This antibody is of value in monitoring the progress of HPV infection from benign lesions to invasive cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for HPV16 E1+E4 Antibody (NB100-2766) (0)

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Secondary Antibodies

 

Isotype Controls

Additional HPV16 E1+E4 Products

HPV16 E1+E4 NB100-2766

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