HPV16 E1+E4 Antibody (HPV16 E1/E4)

Images

 
Immunohistochemistry-Paraffin: HPV16 E1+E4 Antibody (HPV16 E1/E4) [NBP3-07260] - Formalin-fixed, paraffin-embedded human Cervix stained with HPV16 E1+E4 Mouse Monoclonal Antibody (HPV16 E1/E4).
Immunohistochemistry-Paraffin: HPV16 E1+E4 Antibody (HPV16 E1/E4) [NBP3-07260] - Formalin-fixed, paraffin-embedded human Cervix stained with HPV16 E1+E4 Mouse Monoclonal Antibody (HPV16 E1/E4).

Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications IHC-P
Clone
HPV16 E1/E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

HPV16 E1+E4 Antibody (HPV16 E1/E4) Summary

Immunogen
Recombinant human HPV16 E1+E4 fragment (aa 36-41).
Localization
Nuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Type 16 of Human Papilloma Virus (HPV-16).

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08319)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for HPV16 E1+E4 Antibody (HPV16 E1/E4)

  • ATP dependent helicase E1
  • E1
  • E4
  • HPV 16 ATP dependent helicase E1
  • HPV 16 E1
  • HPV 16 E4
  • HPV 16 replication protein E1
  • HPV16 ATP dependent helicase E1
  • HPV16 E1
  • HPV16 E4
  • HPV16 replication protein E1
  • Human papilloma virus type 16 ATP dependent helicase E1
  • Human papilloma virus type 16 E1
  • Human papilloma virus type 16 E4
  • Human papilloma virus type 16 replication protein E1
  • Human papilloma virus type 16
  • Human papillomavirus type 16 ATP dependent helicase E1
  • Human papillomavirus type 16 E1
  • Human papillomavirus type 16 E4
  • Human papillomavirus type 16 replication protein E1
  • Human papillomavirus type 16
  • Replication protein E1
  • Replication protein

Background

HPV16 is an ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. HPV16, in comparison to HPV types 6 and 11, is more often associated with malignant genital cancers in humans. This antibody is of value in monitoring the progress of HPV infection from benign lesions to invasive cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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    Secondary Antibodies

     

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