![Western Blot: HMW E.Coli Host Cell Proteins Antibody [NBP2-44206] - 2D Western Blot of anti-E.coli High Molecular Weight Host Cell Protein antibody. Load: 35 Ug HMW HCP.](http://images.novusbio.com/fullsize/HMW-E.Coli-Host-Cell-Proteins-Antibody-Western-Blot-NBP2-44206-img0002.jpg "Western Blot: HMW E.Coli Host Cell Proteins Antibody [NBP2-44206] - 2D Western Blot of anti-E.coli High Molecular Weight Host Cell Protein antibody. Load: 35 Ug HMW HCP.")
| Reactivity | EcSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | HMW Host Cell Protein A purified antibody is directed against high molecular weight E.coli Host Cell Proteins. The product was purified from monospecific antiserum by Protein A affinity chromatography. Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | HMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture high molecular weight (HMW) proteins from E.Coli (BL21). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product detects over 250 E.coli HMW proteins and has been tested in 2D-Page, western blotting, and ELISA. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![ELISA: HMW E.Coli Host Cell Proteins Antibody [NBP2-44206] - ELISA of Rabbit Anti-E.coli High Molecular Weight Host Cell Protein Antigen: Total HCP protein. Coating amount: 0.1 Ug per well.](http://images.novusbio.com/fullsize/HMW-E.Coli-Host-Cell-Proteins-Antibody-ELISA-NBP2-44206-img0001.jpg "ELISA: HMW E.Coli Host Cell Proteins Antibody [NBP2-44206] - ELISA of Rabbit Anti-E.coli High Molecular Weight Host Cell Protein Antigen: Total HCP protein. Coating amount: 0.1 Ug per well.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")