HLA-ABCE Antibody (TP25.99SF) - BSA Free

Flow Cytometry: HLA-ABCE Antibody (TP25.99SF) [NBP2-68006] - Staining of human peripheral blood cells using anti-HLA-ABCE (TP25.99SF) purified, GAM-APC.

• Reactivity: Hu
• Applications: WB, Flow, IHC
• Clone: TP25.99SF
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HLA-ABCE Antibody (TP25.99SF) - BSA Free Summary

• Description: Novus Biologicals Mouse HLA-ABCE Antibody (TP25.99SF) - BSA Free (NBP2-68006) is a monoclonal antibody validated for use in IHC, WB and Flow. Anti-HLA-ABCE Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: IFN-gamma-treated human melanoma cells COLO 38.
• Specificity: The mouse monoclonal antibody TP25.99SF recognizes HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for distinguishing between HLA-G and other HLA-class I antigens.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot 1-2 µg/m
• Application Notes: Western blotting: Recommended dilution: 1-2 ug/ml; non-reducing conditions.

Packaging, Storage & Formulations

• Storage: Store at 4°C.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HLA-ABCE Antibody (TP25.99SF) - BSA Free

• HLA-ABC
• HLA-E
• major histocompatibility complex, class I, A
• major histocompatibility complex, class I, B
• major histocompatibility complex, class I, C
• major histocompatibility complex, class I, E
• MHC class I

Background

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g. HLA-E, HLA-G.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLA-ABCE Antibody (NBP2-68006)(1)

Publication using NBP2-68006

• Morosky S, Evans A, Lemon K et al. The neonatal Fc receptor is a pan-echovirus receptor J. Cell. Physiol. 2018-10-28 [PMID: 30808762]