HLA-ABCE Antibody (TP25.99SF)

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Flow Cytometry: HLA-ABCE Antibody (TP25.99SF) [NBP2-68006] - Staining of human peripheral blood cells using anti-HLA-ABCE (TP25.99SF) purified, GAM-APC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC-Fr
Clone
TP25.99SF
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

HLA-ABCE Antibody (TP25.99SF) Summary

Immunogen
IFN-gamma-treated human melanoma cells COLO 38.
Specificity
The mouse monoclonal antibody TP25.99SF recognizes HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for distinguishing between HLA-G and other HLA-class I antigens.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
>95%, by SDS-PAGE
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunohistochemistry-Frozen

Packaging, Storage & Formulations

Storage
Store at 4°C.
Buffer
PBS
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for HLA-ABCE Antibody (TP25.99SF)

  • HLA-ABC
  • HLA-E
  • major histocompatibility complex, class I, A
  • major histocompatibility complex, class I, B
  • major histocompatibility complex, class I, C
  • major histocompatibility complex, class I, E
  • MHC class I

Background

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g. HLA-E, HLA-G.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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