Histone Nucleosome Core Antibody - BSA Free

• Reactivity: Bv
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Format: BSA Free

Histone Nucleosome Core Antibody - BSA Free Summary

• Immunogen: Intact calf H-3 complexed to rRNA.
• Specificity: This is specific for Histone-Core. The antibody binds to H1, H2B, H3 and H4. There is no evidence to suggest that the antibody can bind to H2A.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Immunoprecipitaion, Western Blot. Although not tested this antibody may be useful in Immunoflourescence.

Reactivity Notes

Although not tested this antibody may react with Human and other species.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Purity: Protein G purified

Background

In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. The histone core protein is an octamer consisting of 2 subunits of each of histone 2A, 2B, 3 and 4. In conjunction with the linker histone H1 and the nonhistone chromosomal proteins, it is responsible for the binding and compacting of DNA into chromatin. The sequences of the core histones are highly conserved from species to species.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histone Nucleosome Core Antibody (NB100-745)(1)

Publication using NB100-745

• Balasubramanian N, James TD, Selvakumar GP et al. Repeated ethanol exposure and withdrawal alters angiotensin-converting enzyme 2 expression in discrete brain regions: Implications for SARS-CoV-2 neuroinvasion Alcoholism: Clinical and Experimental Research 2023-02-01 [PMID: 36529893]