Histamine Antibody

Immunocytochemistry: Histamine Antibody [NBP2-45266] - Formalin fixed, paraffin-embedded Rat stomach tissue sections were stained with 1:100 Anti-Histamine antibody produced in Rabbit, followed by 25 ug/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat and by 25 ug/mL ExtrAvidin-Peroxidase.

Immunohistochemistry: Histamine Antibody [NBP2-45266] - Formalin fixed, paraffin-embedded Rat stomach tissue sections were stained with 1:200 Anti-Histamine antibody produced in Rabbit (Cat. No. H7403), followed by 25 ug/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat and by 25 ug/mL ExtrAvidin-Peroxidase.

• Reactivity: Hu, All
• Applications: DB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Histamine Antibody Summary

• Description: Novus Biologicals Rabbit Histamine Antibody (NBP2-45266) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-Histamine Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Histamine conjugated to succinylated KLH.
• Specificity: The product specifically stains histamine-containing cells in paraformaldehyde/carbodiimide-fixed or paraformaldehyde-fixed, paraffin-embedded sections of rat stomach (endocrine cells and mast cells).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:14000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100 - 1:200
• Application Notes: Dot Blot: 1:14,000 using histamine-BSA.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 30758643).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and 1.0% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Histamine Antibody

• 2 (4 imidazolyl)ethylamine
• 4 (2 aminoethyl) 1 3 diazole

Background

Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. Histamine causes running nose, sneezing and itching, and narrows the airways in the lungs. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histamine Antibody (NBP2-45266)(2)

Publications using NBP2-45266

• McAdams J, Ebott J, Jansen C et al. Neoadjuvant chemotherapy induces phenotypic mast cell changes in high grade serous ovarian cancer. Journal of ovarian research 2024-09-29 [PMID: 39342316]
• Xiong Y, Liu L, Xia Y et al. Tumor infiltrating mast cells determine oncogenic HIF-2a-conferred immune evasion in clear cell renal cell carcinoma Cancer Immunol. Immunother. 2019-02-13 [PMID: 30758643] (IHC-P, Human)