HIST1H2AB Antibody (1H12) - BSA Free

Western Blot: HIST1H2AB Antibody (1H12) [NBP3-15228] - Positive WB detected in HepG2 whole cell lysate. All lanes: HIST1H2AB antibody at 2.7ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 13 KDa. Observed band size: 13 Kda.

Immunocytochemistry/Immunofluorescence: HIST1H2AB Antibody (1H12) [NBP3-15228] - Immunofluorescence staining of Hela cells with at HIST1H2AB antibody 1:168, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C.The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1H12
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HIST1H2AB Antibody (1H12) - BSA Free Summary

• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide. UniProt ID (P04908).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: H2AC4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HIST1H2AB Antibody (1H12) - BSA Free

• histone H2A type 1-B/E
• H2A/m
• H2AFM
• histone cluster 1, H2ab

Background

HIST1H2AB is 130 amino acids long, weighs approximately 14 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including riddle syndrome, systemic lupus erythematosus, lupus erythematosus, cockayne syndrome, hemochromatosis, and immunodeficiency. HIST1H2AB has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the proteasome degradation, telomere maintenance, nucleosome assembly, cell cycle, and systemic lupus erythematosus pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2AC4