Hexokinase 1/2 Antibody (927312) [Unconjugated]


Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Hexokinase 1/2 ...read more
Hexokinase 1/2 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human Hexokinase 1/2 Monoclonal Antibody (Catalog # MAB8179) at 15 µg/mL overnight at 4 °C. ...read more
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Hexokinase 1/2 at approximately 96 kDa (as indicated) using 5 ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Hexokinase 1/2 Antibody (927312) [Unconjugated] Summary

E. coli-derived recombinant human Hexokinase 2.
Accession # P52789
Detects human Hexokinase 2 in direct ELISAs. Detects human Hexokinase 1 and human Hexokinase 2 in Western blots.
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 8-25 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.2 ug/mL
Read Publication using
MAB8179 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hexokinase 1/2 Antibody (927312) [Unconjugated]

  • Hexokinase 1/2


Hexokinases phosphorylate hexose to form hexose 6-phosphate, the first step in hexose metabolism (1). Phosphorylation of a hexose adds charge to molecule thereby making it difficult to transport out of a cell. The hexose is therefore retained for intracellular metabolic processes, such as glycolysis or glycogen synthesis. In most organisms, glucose is the most important substrate of hexokinases and glucose-6-phosphate is the most important product. There are four mammalian hexokinases (2). Hexokinase 1, 2 and 3 are referred to as high-affinity hexokinases because their Km for glucose is below 1 mM. Hexokinase 4 is specific for glucose and is also referred to as glucokinase (3). Hexokinase 2 (HK2), also known as muscle form hexokinase, localizes to the outer membrane of mitochondria and is present in adipose tissue, skeletal muscle, and heart (4). The amino acids corresponding to the mitochondrial binding domain (5) have been removed in the recombinant enzyme. Like Hexokinase 1 (HK1), HK2 contains two homologous halves that may have evolved from an ancestral hexokinase through gene duplication and tandem ligation (6). Unlike HK1, in HK2 both the C-terminal and N-terminal portions are catalytically active with the N-terminal half having higher activity than the C-terminal half (7). In HK2 both the N-terminal and C-terminal halves exhibit product inhibition. HK2 overexpression is required for tumor growth making HK2 an attractive oncotarget (4). The enzymatic activity of recombinant human HK2 is measured using a phosphatase-coupled method (8).
  1. Aleshin, A.E. et al. (1998) Structure 6:39-50
  2. Takeda, J. et al. (1993) J. Biol. Chem. 268:15200.
  3. Lange, A.J. et al. (1991) Biochem. J. 277: 159.
  4. Patra, K.C. et al. (2013) Cancer Cell. 24:213.
  5. Bianchi M et al. (1998) Mol Cell Biochem 189:185.
  6. Ureta, T. (1982) Comp Biochem Physiol. B. 71B:549.
  7. Ahn, K.J. et al. (2009) BMB Rep. 42:350.
  8. Wu, Z.L. (2011) PLoS One 6:e23172.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hexokinase 1/2 Antibody (MAB8179)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.

Filter By Application
Western Blot
All Applications
Filter By Species
All Species

Reviews for Hexokinase 1/2 Antibody (MAB8179) (0)

There are no reviews for Hexokinase 1/2 Antibody (MAB8179). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Hexokinase 1/2 Antibody (MAB8179) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional Hexokinase 1/2 Products

Blogs on Hexokinase 1/2

There are no specific blogs for Hexokinase 1/2, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Hexokinase 1/2 Antibody (927312) [Unconjugated] and receive a gift card or discount.