Hexokinase 1/2 Antibody (927312) [DyLight 488]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 927312
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 488

Hexokinase 1/2 Antibody (927312) [DyLight 488] Summary

• Immunogen: E. coli-derived recombinant human Hexokinase 2.
  Phe11-Arg917
  Accession # P52789
• Specificity: Detects human Hexokinase 2 in direct ELISAs. Detects human Hexokinase 1 and human Hexokinase 2 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Hexokinase 1/2 Antibody (927312) [DyLight 488]

• Hexokinase 1/2

Background

An alteration in the permeability of the mitochondrial membrane is a hallmark of the intrinsic pathway of caspase activation. This change in permeability leads to the cytosolic release of multiple proteins, including cytochrome c, Smac/Diablo, HTRA2/Omi, Apoptosis Inducing Factor (AIF), and Endonuclease G from the intermembrane space of the mitochondria. The release of these proteins, which are normally sequestered, has severe consequences for the cell. For example, cytoplasmic cytochrome c and dATP initiate the assembly of the apoptosome with APAF-1 and pro-caspase-9. The assembly of this complex promotes the activation of caspase-9 which subsequently leads to the activation of caspase-3, -6, and -7 to induce apoptosis. In addition, Smac/Diablo and HTRA2/Omi interact with the IAP proteins disrupting their abilities to inhibit caspase activity, while AIF and Endonuclease G translocate to the nucleus upon mitochondrial release and promote chromatin condensation and DNA fragmentation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.