Herpes Simplex Virus VP16 Antibody - BSA Free

Western Blot: Herpes Simplex Virus VP16 Antibody - BSA Free [NB600-950] - Whole extract of 293T cells expressing VP16 fusion proteins were separated on SDS-PAGE and probed with Anti-VP16 antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:2,000 antibody2. Negative Control

• Reactivity: V-Vi
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 5.8 mg/ml

Herpes Simplex Virus VP16 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide corresponding to amino acids 474-487 of the herpes simplex virus Herpes Simplex Virus VP16 protein, conjugated to KLH via an N-terminal added cysteine.
• Specificity: Recognizes Herpes Simplex Virus VP16 fusion protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 10ug
  • Western Blot 1:1000

Reactivity Notes

Tag specific

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 5.8 mg/ml
• Purity: Immunogen affinity purified

Background

VP16 from herpes simple virus (HSV) is a strong transcriptional activator of immediate early viral genes.1 Due to its lack of DNA binding activity, VP16 is recruited to DNA via interaction with DNA binding proteins.2 Ptashne et al. showed that VP16 can function as a potent transcriptional activator in mammalian cells when fused to the DNA binding domain of the yeast transcriptional activator GAL4.3 These properties were taken into consideration in the development of a mammalian version of the Yeast Two-Hybrid System.4-6 The Yeast Two-Hybrid System is a powerful method for characterizing and screening for protein-protein interactions. In the original version of the system, GAL4 DNA binding domain is fused to protein X, and GAL4 activation domain is fused to protein Y. Neither hybrid is capable of activating transcription individually. Upon interaction between proteins X and Y, the DNA binding and activator domains are brought in close proximity. As a result, the transcriptional activity of GAL4 is reconstituted at the promoter, and monitored with a reporter gene.4 Additional two-hybrid systems were developed using different DNA binding and activation domains as well as different reporter genes.5-9 The VP16 activator is used in yeast and mammalian systems. In yeast, its DNA interaction partner is typically LexA.10 In mammalian systems, interaction between two proteins is detected through recruitment of VP16 and GAL4 DNA binding domain to GAL4 operators present in the reporter gene.7,9-11

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.