Human HBsAg ELISA Kit (Colorimetric)

Human HBsAg ELISA Kit (Colorimetric) [NBP2-68136] - Standard Curve Reference

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Human HBsAg ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human HBsAg in samples. No significant cross-reactivity or interference between Human HBsAg and analogues was observed.
• Standard Curve Range: 7.81 - 500 ng/mL
• Sensitivity: 4.69 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.42%
• Intra-Assay: CV% < 4.91%
• Spike Recovery: 84-102%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: S

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human HBsAg ELISA Kit (Colorimetric)

• HBsAg
• HBV major surface antigen
• HBV surface antigen
• Hepatitis B Virus major surface antigen
• Hepatitis B Virus Surface Antigen
• Major surface antigen
• protein S

Background

Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for HBsAg ELISA Kit (NBP2-68136)(1)

Publication using NBP2-68136

• Schmidt S, Mengistu M, Daffis S et al. Alternating arenavirus vector immunization generates robust polyfunctional genotype cross-reactive HBV-specific CD8 T cell responses and high anti-HBs titers The Journal of infectious diseases 2023-08-21 [PMID: 37602681] (ELISA)

FAQs for HBsAg ELISA Kit (NBP2-68136). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: S