HBsAg Antibody (HB5) - BSA Free

• Reactivity: V
• Applications: ELISA, ICC/IF
• Clone: HB5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HBsAg Antibody (HB5) - BSA Free Summary

• Description: Novus Biologicals Mouse HBsAg Antibody (HB5) - BSA Free (NB500-475) is a monoclonal antibody validated for use in ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: purified HbsAg from human plasma
• Specificity: This antibody (clone HB5) recognizes following Hepatitis B virus (HBV) subtypes: ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-. Hepatitis B surface antigen (HBsAg) is a marker of infectivity. Specificity of the antibody HB5 was verified by ELISA on panel of virus subtypes identified on International Workshop on HBsAg Subtypes (Paris, April 1975). The antibody HB5 does not cross-block with This antibody HB3.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: S
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
• Application Notes: ELISA: Use at an assay dependant concentration. ICC: Use at an assay dependant dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Multi-step

Alternate Names for HBsAg Antibody (HB5) - BSA Free

• HBsAg
• HBV major surface antigen
• HBV surface antigen
• Hepatitis B Virus major surface antigen
• Hepatitis B Virus Surface Antigen
• Major surface antigen
• protein S

Background

Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: S