HBcAg Antibody (Hep2 (H69)) - BSA Free

• Reactivity: V-Vi
• Applications: IHC
• Clone: Hep2 (H69)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.25 mg/ml

HBcAg Antibody (Hep2 (H69)) - BSA Free Summary

• Description: Novus Biologicals Mouse HBcAg Antibody (Hep2 (H69)) - BSA Free (NB100-64454) is a monoclonal antibody validated for use in IHC. Anti-HBcAg Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified hepatitis B core antigen
• Specificity: NB100-64454 is specific for Hepatitis B core antigen (HBcAg).
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: C
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Application Notes: Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 30408957).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: <0.1% Sodium Azide
• Concentration: 0.25 mg/ml
• Purity: Affinity purified

Alternate Names for HBcAg Antibody (Hep2 (H69)) - BSA Free

• c
• Core antigen
• HBc
• HBcAg
• Hepatitis B Virus core antigen

Background

Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection. This antibody stains predominantly the nuclei of liver cells. Occasionally variable staining may also be seen in the perinuclear cytoplasm. Hepatitis B core antigen (HBcAg), the highly immunogenic 21kDa replicative form of the Hepatitis B virus (HBV), expressed by infected cells. Antibodies to HBcAg are produced by the host during and following an acute HBV infection and can be detected in chronic HBV carriers and patients recovered from HBV infection, usually persisting in the body for life.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HBcAg Antibody (NB100-64454)(1)

Publication using NB100-64454

• Ye X, Tateno C, Thi EP et al. Hepatitis B Virus Therapeutic Agent ARB-1740 Has Inhibitory Effect on Hepatitis Delta Virus in a New Dually-Infected Humanized Mouse Model. ACS Infect Dis. 2018-11-20 [PMID: 30408957] (IHC-P, Virus)

FAQs for HBcAg Antibody (NB100-64454). (Showing 1 - 2 of 2 FAQ).

1. Is it possible to help me double confirm if these products contain any BSA? Catalog numbers: NB400-105, NB100-306, and NB100-64454.
   • No, none of these antibodies have BSA in their formulation.
2. We are interested in your HBc Ab (Cat# NB100-64454). Would you please provide the epitope of amino acid sequence of this Ab? The datasheet do not mention this information.
   • This monoclonal antibody was raised against the purified hepatitis B core antigen (HBcAg, 1-183 a.a.). According to our database, HBcAg is the highly immunogenic 21 kDa replicative form of the Hepatitis B virus (HBV), expressed by infected Hep2 cells. Unfortunately, our lab has not mapped the epitope for this monoclonal antibody; therefore, there is not such information I can provide to you. Other available information about this clone is related to how it was produced and purified, for your reference: Spleen cells from immunized Balb/C mice were fused with cells of the SP2/0-Ag-14 myeloma cell line. Purified IgG was prepared by affinity chromatography and refined by S300 exclusion chromatography.

Bioinformatics

• Gene Symbol: C