![Western Blot: GRAMD1A Antibody [NBP2-32148] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, MCF7, Jurkat, and K562 cells. Antibodies: Affinity purified rabbit anti-GRAMD1A antibody NBP2-32148 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GRAMD1A-Antibody-Western-Blot-NBP2-32148-img0001.jpg "Western Blot: GRAMD1A Antibody [NBP2-32148] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, MCF7, Jurkat, and K562 cells. Antibodies: Affinity purified rabbit anti-GRAMD1A antibody NBP2-32148 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen this antibody was made to, maps to a region between residue 674 to 724 of human GRAM Domain Containing 1A using the numbering given in entry NP_065946.2 (GeneID 57655). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GRAMD1A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP2-32148 | Applications | Species |
|---|---|---|
| Ogasawara F, Ueda K ATP-binding cassette A1 and cholesterol transfer protein Aster-A promote an asymmetric cholesterol distribution in the plasma membrane The Journal of biological chemistry 2022-11-14 [PMID: 36395885] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | GRAMD1A |
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![Immunoprecipitation: GRAMD1A Antibody [NBP2-32148] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GRAMD1A antibody NBP2-32148 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated GRAMD1A, NBP2-32148 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GRAMD1A-Antibody-Immunoprecipitation-NBP2-32148-img0002.jpg "Immunoprecipitation: GRAMD1A Antibody [NBP2-32148] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GRAMD1A antibody NBP2-32148 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated GRAMD1A, NBP2-32148 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")