GPR108 Antibody (1047612) [Unconjugated]

GPR108 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human GPR108 Monoclonal Antibody (Catalog # MAB11128) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm in glandular epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western blot shows lysates of HEK293T human embryonic kidney cell line (wild type) and HEK293T human embryonic kidney cell line transfected with human GPR108. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GPR108 Monoclonal Antibody (Catalog # MAB11128) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GPR108 at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 1047612
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GPR108 Antibody (1047612) [Unconjugated] Summary

• Immunogen: Human GPR108 synthetic peptide
  Accession # Q9NPR9
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR108 Antibody (1047612) [Unconjugated]

• G protein-coupled receptor 108
• GPR108
• LUSTR2
• LUSTR2Lung seven transmembrane receptor 2
• MGC14393
• protein GPR108

Background

Human GPR108, also known as Lung seven transmembrane receptor 2, is a 543 amino acids long receptor belonging to the G-protein-coupled receptors (GPCRs) family of receptors. Human GPR108 may play a role in intracellular immune modulation by activating NF-kappaB response and attenuating Toll-like-receptor response (1). I has also been shown essential function in adeno-associated virus (AAV) entry to cells (2).

1. Dong D. et al. PLoS One. 2018 Oct 17;13(10):e0205303
2. Dudek AM. et al. Mol Ther. 2020 Feb 5;28(2):367-381.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for GPR108 Antibody (MAB11128). (Showing 1 - of FAQ).

Bioinformatics

• Uniprot:
  • Q9NPR9(Human)