GNB5 Overexpression Lysate (Native)


Western Blot: GNB5 Overexpression Lysate (Adult Normal) [NBL1-11169] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GNB5.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

GNB5 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC204134 and based on accession number NM_006578. The protein contains a C-terminal DDK tag.
Homo sapiens guanine nucleotide binding protein (G protein), beta 5 (GNB5), transcript variant 1, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
38.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for GNB5

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GNB5 Overexpression Lysate (Native)

  • FLJ37457
  • FLJ43714
  • G protein, beta subunit 5L
  • G protein, beta-5 subunit
  • GB5
  • gbeta5
  • guanine nucleotide binding protein (G protein), beta 5
  • guanine nucleotide-binding protein subunit beta-5
  • guanine nucleotide-binding protein, beta subunit 5L
  • Transducin beta chain 5


Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternatively spliced transcript variants encoding different isoforms exist. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the shorter isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Gene Symbol GNB5