GM2 Ganglioside Antibody (L55-81) - Chimeric

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Immunocytochemistry/ Immunofluorescence: GM2 Ganglioside Antibody (L55-81) - Chimeric [NBP2-81278] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells on Shi-fix coverslips, permeabilized with 0.15% ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Func, ICC/IF, IHC, Cell Depl
Clone
L55-81
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

GM2 Ganglioside Antibody (L55-81) - Chimeric Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
L55-81 was prepared by isolating EBV-immortalized peripheral blood B lymphocytes from a human donor with melanoma.
Specificity
L55-81 binds specifically to GM2 ganglioside; this antibody shows no reactivity towards GM1, GM3, GD2 or normal tissues- the L55-81 epitope contains both a GalNAc and sialic acid bound to lactose. Ganglioside GM2 (GalNAcbeta1-4(NeuNAcalpha2-3)Galbeta1-4Glcbeta-1ceramide) is a glycosphingolipid present on the cell surface and a major tumor-differentiation antigen in human melanoma and hepatoma.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Cell depletion
  • ELISA
  • Functional
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques. L55-81 reacts positively to melanoma cell lines with high GM2 expression (M14; M24), and shows no binding to normal cell types or cell lines M15 or M25 which have low GM2 expression and high GM3 expression. This specificity can be confirmed by ELISA and TLC immunostaining by testing a variety of gangliosides and neutral glycolipids. L55-81 is able to lyse cells from the M14 melanoma cell line (which has high GM2 expression) in the presence of human or rabbit complement, but not in the absence of complement - this was shown by a [51Cr] release assay. Biotinylated L55-81 has been used for IHC of acetone-fixed sections of tumor tissue in an avidin-biotin complex immunoperoxidase system. Statistically significant positive staining (determined by Fisher's exact test) was displayed with melanoma, colon carcinoma and ovary carcinoma tumor tissues. Staining has also be observed with breast carcinoma, prostate carcinoma and kidney carcinoma.

Packaging, Storage & Formulations

Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Buffer
PBS
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for GM2 Ganglioside Antibody (L55-81) - Chimeric

  • GM2 (ganglioside)
  • Tay-Sachs ganglioside

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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