![Immunocytochemistry /Immunofluorescence: GM2 Ganglioside Antibody (KM966) - Chimeric - BSA Free [NBP3-43552] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with NBP3-43552 (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43552, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (NBP2-52638) followed by staining with Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43552_rabbit-gm2-ganglioside-mab-km966-chimeric-azide-bsa-free-74202522113015.png "Immunocytochemistry /Immunofluorescence: GM2 Ganglioside Antibody (KM966) - Chimeric - BSA Free [NBP3-43552] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with NBP3-43552 (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43552, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (NBP2-52638) followed by staining with Alexa Fluor 488 secondary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF |
| Clone | KM966 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This chimeric antibody was generated by cloning the variable heavy and light chain sequences of murine parent antibody KM696 (IgM) and expressing it as a mouse/human IgG1. The parent antibody was raised against human GM2. |
| Specificity | This antibody recognizes and binds ganglioside GM2. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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