Glucose Oxidase Antibody - BSA Free

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Western Blot: Glucose Oxidase Antibody [NBP2-44232] - lane 1: Glucose Oxidase. Lane 2: None. Load: 50 ng per lane.

Product Details

Summary
Reactivity FuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
LYOPH

Order Details

Glucose Oxidase Antibody - BSA Free Summary

Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Immunogen
Glucose Oxidase Antibody was produced by repeated immunizations with Aspergillus niger Glucose Oxidase protein. (Uniprot: P13006)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Multi-step
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Applications/Dilutions

Dilutions
  • ELISA 1:35000-1:160000
  • Immunohistochemistry 1:2000-1:8000
  • Western Blot 1:3500-1:16000
Application Notes
This product has been tested by ELISA and western blot and is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.

Reactivity Notes

Reactive with Glucose Oxidase from Aspergillus niger.
Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Glucose Oxidase Antibody - BSA Free

  • Beta D Glucose Oxygen 1 Oxido Reductase
  • Glucose Oxyhydrase
  • GOD

Background

Glucose Oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose or oxygen from foods and beverages. Glucose Oxidase producing moulds such as Aspergillus and Penicillium species are used for the biological production of gluconic acid. CATALYTIC ACTIVITY: Beta D glucose + O2 = D glucono 1,5 lactone + H2O2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

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