GFP Antibody

Images

 
Western Blot: GFP Antibody [NB600-310] - WB validation of GFP antibody on (1) HeLa whole cell lysateand (2) GFP transfected HeLa whole cell lysate. The primary antibody was used at 1:2000 dilution and the signal was ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-310] - HEK 293 cells expressing GFP were visualized for GFP fluorescence (Green) or using NB600-310 to label the GFP (Red). Nuclei (Blue) were counterstained ...read more
Western Blot: GFP Antibody [NB600-310] - Detection of GFP in Western blot using NB 600-310. Lane 1: A549 + 500 ng GFP. Lane 2: A549 + 100 ng GFP. Lane 3: A549 + 20 ng GFP. Lane 4: 20 ng GFP. 10 ug of lysate were loaded ...read more
Simple Western: GFP Antibody [NB600-310] - Simple Western lane view shows a specific band for GFP in 1.25 mg/ml of Recombinant EGFP (left) and rhSNAP25-GFP (right) lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Product Discontinued
View other related GFP Primary Antibodies

Order Details


    • Catalog Number
      NB600-310
    • Availability
      Product Discontinued

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GFP Antibody Summary

Immunogen
Highly purified native GFP Aequorea victoria.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Simple Western 1:50
  • ELISA 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Application Notes
This GFP antibody is useful for Immunocytochemistry/Immunofluorescence, Western blot and ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Publications
Read Publications using
NB600-310 in the following applications:

Reactivity Notes

Reacts against all variants of recombinant Aequorea GFP. However, there is little to no reactivity with Renilla GFP.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
10mM Tris (pH 8.0)
Preservative
0.02% Sodium Azide
Concentration
0.25 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GFP Antibody

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

GFP (green fluorescent protein) is a fluorescent protein produced by the crystal jellyfish, Aequorea victoria; which produces glowing points of light around its umbrella's margins through bioluminescence reactions in photogenic cells. GFP gene has been isolated and has emerged as a useful tool for making expressed proteins fluorescent by generating chimeric genes composed of those of GFP/its different colour variants linked to genes of target proteins. GFP is now a widely used reporter protein in eukaryotic/prokaryotic gene expression studies, and as a protein tag in tissue culture as well as multicellular organisms for localizing and studying protein movement/dynamics in cells or tissues. GFP epitope tags are useful for labeling/detection of proteins using various techniques including immunodetection methods and GFP does not affect the tagged protein's biochemical properties. GFP absorbs light maximally at 395 nm with an emission spectrum that peaks at 509 nm and has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB600-310)(2)

We have publications tested in 1 confirmed species: Firefly.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
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Filter By Species
Firefly
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFP Antibody (NB600-310). (Showing 1 - of FAQs).

Secondary Antibodies

 

Isotype Controls

Additional GFP Products

Research Areas for GFP Antibody (NB600-310)

Find related products by research area.

Blogs on GFP.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

GFP - Be Green!
Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

GFP (Green Fluorescent Protein) Bringing Light to Life
GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

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