Western Blot: GFP Antibody [NB600-310] - WB validation of GFP antibody on (1) HeLa whole cell lysateand (2) GFP transfected HeLa whole cell lysate. The primary antibody was used at 1:2000 dilution and the signal was ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-310] - HEK 293 cells expressing GFP were visualized for GFP fluorescence (Green) or using NB600-310 to label the GFP (Red). Nuclei (Blue) were counterstained ...read more
Western Blot: GFP Antibody [NB600-310] - Detection of GFP in Western blot using NB 600-310. Lane 1: A549 + 500 ng GFP. Lane 2: A549 + 100 ng GFP. Lane 3: A549 + 20 ng GFP. Lane 4: 20 ng GFP. 10 ug of lysate were loaded ...read more
Simple Western: GFP Antibody [NB600-310] - Simple Western lane view shows a specific band for GFP in 1.25 mg/ml of Recombinant EGFP (left) and rhSNAP25-GFP (right) lysate. This experiment was performed under reducing ...read more
Reacts against all variants of recombinant Aequorea GFP. However, there is little to no reactivity with Renilla GFP.
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
10mM Tris (pH 8.0)
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for GFP Antibody
Enhanced Green Fluorescent Protein
green fluorescent protein (gfp)
Green Fluorescent Protein
GFP (green fluorescent protein) is a fluorescent protein produced by the crystal jellyfish, Aequorea victoria; which produces glowing points of light around its umbrella's margins through bioluminescence reactions in photogenic cells. GFP gene has been isolated and has emerged as a useful tool for making expressed proteins fluorescent by generating chimeric genes composed of those of GFP/its different colour variants linked to genes of target proteins. GFP is now a widely used reporter protein in eukaryotic/prokaryotic gene expression studies, and as a protein tag in tissue culture as well as multicellular organisms for localizing and studying protein movement/dynamics in cells or tissues. GFP epitope tags are useful for labeling/detection of proteins using various techniques including immunodetection methods and GFP does not affect the tagged protein's biochemical properties. GFP absorbs light maximally at 395 nm with an emission spectrum that peaks at 509 nm and has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for GFP Antibody (NB600-310)(3)
We have publications tested in 1 confirmed species: Firefly.
We have publications tested in 2 applications: ICC/IF, WB.
GFP - Be Green! Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v... Read full blog post.
Epitope Tags and the V5 Tag Antibody We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r... Read full blog post.
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