![Immunocytochemistry/Immunofluorescence: GCN4 Antibody (C11L34) [NBP2-81274] - Immunofluorescence staining of fixed HeLa cells expressing mCherry tagged GCN4 with anti-GCN4 antibody (C11L34). Immunofluorescence analysis of fixed HeLa cells stained with the chimeric rabbit IgG version of C11L34 [NBP2-81274] followed by an Alexa Fluor 488 secondary antibody, showing mitochondrial staining. Colocalisation with mCherry tagged GCN4 demonstrates the very low off target/non-specific binding of this antibody. The nuclear and mitochondrial DNA stain is hoechst (blue). Panels show; a) mCherry tagged, mitochondrially targetted GCN4, b) NBP2-81274 with Alexa Fluor® 488 secondary antibody, c) DNA stained with hoechst, d) Merged channels. Images courtesy of Luming Chen (Kim Orth lab, UT Southwestern Medical Center).](http://images.novusbio.com/fullsize/GCN4-Antibody-C11L34-Immunocytochemistry-Immunofluorescence-NBP2-81274-img0001.jpg "Immunocytochemistry/Immunofluorescence: GCN4 Antibody (C11L34) [NBP2-81274] - Immunofluorescence staining of fixed HeLa cells expressing mCherry tagged GCN4 with anti-GCN4 antibody (C11L34). Immunofluorescence analysis of fixed HeLa cells stained with the chimeric rabbit IgG version of C11L34 [NBP2-81274] followed by an Alexa Fluor 488 secondary antibody, showing mitochondrial staining. Colocalisation with mCherry tagged GCN4 demonstrates the very low off target/non-specific binding of this antibody. The nuclear and mitochondrial DNA stain is hoechst (blue). Panels show; a) mCherry tagged, mitochondrially targetted GCN4, b) NBP2-81274 with Alexa Fluor® 488 secondary antibody, c) DNA stained with hoechst, d) Merged channels. Images courtesy of Luming Chen (Kim Orth lab, UT Southwestern Medical Center).")
| Reactivity | YeSpecies Glossary |
| Applications | WB, ICC/IF, IP, SPR |
| Clone | C11L34 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Biotinylated GCN4-peptide (HLENEVARLKK). |
| Specificity | This antibody binds to the HLENEVARLKK peptide from the leucine-zipper domain of the S. cerevisiae GCN4 transcriptional activator with picomolar affinity. |
| Isotype | IgG Lambda |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | GCN4 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to the GCN4-leucine zipper peptide, which is part of the dimerisation- and DNA-binding domain of the transcriptional activator GCN4 in S. cerevisiae. |
| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
