![Immunocytochemistry/ Immunofluorescence: Ganglioside GD2 Antibody (14G2a) - Chimeric - BSA Free [NBP3-43551] - Immunofluorescence analysis of paraformaldehyde fixed Kelly cells on coverslips stained with NBP3-43551 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43551, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43551_rabbit-ganglioside-gd2-mab-14g2a-chimeric-azide-bsa-free-immunocytochemistry-immunofluorescence-222026035118.png "Immunocytochemistry/ Immunofluorescence: Ganglioside GD2 Antibody (14G2a) - Chimeric - BSA Free [NBP3-43551] - Immunofluorescence analysis of paraformaldehyde fixed Kelly cells on coverslips stained with NBP3-43551 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43551, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow |
| Clone | 14G2a |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | The original murine parent antibody was generated against human neuroblastoma cell line LAN-1. This IgG2a isotype version was then generated by class switching of the murine parent antibody 14.18 (IgG3). |
| Specificity | This antibody binds the GD2 antigen and GD2 expressing neuroblastoma cell lines with an affinity similar to the parent antibody. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: Ganglioside GD2 Antibody (14G2a) - Chimeric - BSA Free [NBP3-43551] - Kelly cells were stained with the anti-unknown specificity antibody (isotype control, black line) or NBP3-43551 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-43551_rabbit-ganglioside-gd2-mab-14g2a-chimeric-azide-bsa-free-flow-cytometry-2220260325824.png "Flow Cytometry: Ganglioside GD2 Antibody (14G2a) - Chimeric - BSA Free [NBP3-43551] - Kelly cells were stained with the anti-unknown specificity antibody (isotype control, black line) or NBP3-43551 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")