Fractin Antibody

Western Blot: Fractin Antibody [NBP2-29504] - Western blot of colchicine treated Sy5y cell lysate showing specific immunolabeling of the ~ 32k cleaved actin fragment (fractin).

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Fractin Antibody Summary

• Description: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
• Immunogen: Synthetic peptide corresponding to amino acid residues from the C terminal region of the 32-kDa actin fragment
• Specificity: Specific for the ~ 32 kDa fractin protein in Western blots with no reactivity to intact actin. There is often a ladder of smaller bands in cells or culture or in vivo preparations due to further degradation by other proteases.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:100
  • Western Blot 1:1000
• Application Notes: The band of interest is located at ~32 kDa. There is often a ladder of smaller bands in cells or culture or in vivo preparations due to further degradation by other proteases.
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Neat whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for Fractin Antibody

• Anti-Fractin
• fragment of actin

Background

Fractin (fragment of actin) is a caspase-specific cleavage product of actin and serves as a novel marker of apoptosis-related events. The antibody has been shown to detect the processes and cell bodies of degenerating neurons and plaque-associated microglia in Alzheimer's disease (Yang et al., 1998). It has recently been reported that Fractin may have a functional role in apoptotic signaling in oligodendrocytes (Schulz, R., et al., Glia, 2009, in press).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.