FOLR4 Antibody

Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human FOLR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6328) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FOLR4 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

FOLR4 Antibody Summary

• Immunogen: E. coli-derived recombinant human FOLR4
  Glu134-Asn210
  Accession # NP_001073955
• Specificity: Detects human FOLR4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FOLR1, rhFOLR2, and rhFOLR3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: FOLR4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FOLR4 Antibody

• folate receptor 4 (delta) homolog (mouse)
• Folbp3
• FOLR4
• FR-delta
• LOC390243
• probable folate receptor delta

Background

FOLR4 (Folate receptor 4; also FR4 and folate receptor δ and folate binding protein 3) is a 34‑36 kDa member of the folate receptor family of molecules. Based on mouse, it appears to be a marker for subsets of T cells. In particular, and in conjunction with CD25, FR4^hiCD25^hi expression defines T reg cells, FR4^loCD25⁺ expression pattern identifies effector memory T cells, and a FR4^hiCD25^- combination characterizes central memory T cells. Mature human FOLR4 is 225 amino acids (aa) in length (aa 20‑244). It contains virtually no identifiable motifs. There is one potential splice variant that shows a five aa substitution for aa 111‑116. Over aa 134‑220, human FOLR4 shares 72% aa identity with mouse FOLR4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOLR4
• Uniprot:
  • NP_001073955()