FOLR4 Antibody (821221) [Unconjugated]

FOLR4 was detected in immersion fixed paraffin-embedded sections of human melanoma using Rat Anti-Human FOLR4 Monoclonal Antibody (Catalog # MAB6328) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 821221
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

FOLR4 Antibody (821221) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human FOLR4
  Glu134-Trp210
  Accession # A6ND01
• Specificity: Detects human FOLR4 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: FOLR4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FOLR4 Antibody (821221) [Unconjugated]

• folate receptor 4 (delta) homolog (mouse)
• Folbp3
• FOLR4
• FR-delta
• LOC390243
• probable folate receptor delta

Background

FOLR4 (Folate Receptor 4), also known as FR4, Folate Receptor δ, and Folate Binding Protein 3, is a 34‑36 kDa member of the folate receptor family of molecules. Based on mouse, it appears to be a marker for subsets of T cells. In particular, and in conjunction with CD25, FR4^hiCD25^hi expression defines T reg cells, FR4^loCD25⁺ expression pattern identifies effector memory T cells, and a FR4^hiCD25^- combination characterizes central memory T cells. Mature human FOLR4 is 225 amino acids (aa) in length (aa 20‑244). It contains virtually no identifiable motifs. There is one potential splice variant that shows a five aa substitution for aa 111‑116. Over aa 134‑220, human FOLR4 shares 72% aa identity with mouse FOLR4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FOLR4 Antibody (MAB6328)(1)

Publication using MAB6328

• J Vondrakova, M Frolikova, L Ded, J Cerny, P Postlerova, V Palenikova, O Simonik, Z Nahacka, K Basus, E Valaskova, R Machan, A Pacey, Z Holubcova, P Koubek, Z Ezrova, S Park, R Liu, R Partha, N Clark, J Neuzil, M Ikawa, K Erickson, KS Lam, H Moore, K Komrskova MAIA, Fc receptor-like 3, supersedes JUNO as IZUMO1 receptor during human fertilization Science Advances, 2022-09-07;8(36):eabn0047. 2022-09-07 [PMID: 36070373] (Binding Assay, Immunoprecipitation, Western Blot, Human)

Bioinformatics

• Gene Symbol: FOLR4
• Uniprot:
  • A6ND01(Human)