Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2))

Images

 
Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [NBP2-52709] - Human C3/36 cell line. Image from verified customer review.
Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [NBP2-52709] - Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [PE] [NBP2-52709PE] - Mk2 cell line was infected with ZIKA virus ...read more
ELISA: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [NBP2-52709] - ELISA on Denge Virus-Like Particles using NBP2-52709. Dengue Virus Serotype 2 VLPs were coated onto a plate at 5ug/ml. Anti-flavivirus antibody ...read more
Binding Activity: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [NBP2-52709] - IF assay in a high throughput format for screening Dengue virus inhibitors. HEK 293 cells in DMEM 5% FBS/1% PS at 5000 cells/well ...read more

Product Details

Summary
Reactivity Pm, V-ViSpecies Glossary
Applications WB, ELISA, Flow, Func, ICC/IF, IHC, IHC-P, Binding Activity, CyTOF-ready, ICC/IF
Clone
D1-4G2-4-15 (4G2)
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This recombinant Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) was prepared from Dengue Virus type 2 antigens.
Specificity
This Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) recognises flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Functional
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Binding Activity
  • CyTOF-ready
  • Immunofluorescence 1:10 - 1:500
Publications
Read Publications using
NBP2-52709 in the following applications:

Reactivity Notes

Dengue Virus, Zika Virus, West Nile Virus, Yellow Fever Virus, Flaviviridae. Monkey reactivity reported in scientific literature (PMID:33027261).

Packaging, Storage & Formulations

Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Buffer
PBS
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2))

  • Envelope Protein
  • Protein E

Background

Flaviviruses are a group of seventy enveloped, positive-sense single stranded RNA viruses belonging to the Flaviviridae family (1-5). Flaviviruses are typically transmitted through arthropods, like mosquitoes and ticks, and can infect humans causing outbreaks and serious illness or death worldwide (1-5). These well-known viral pathogens include dengue virus (DENV), West Nile virus (WNV), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBEV), Yellow Fever virus (YFV), and Zika virus (ZIKV) (1-5). Flaviviruses consist of ~10.7 kb RNA genome with one open reading frame (ORF) that is flanked by 5' and 3' untranslated regions (UTRs) (1, 2, 6). The ORF encodes for a polyprotein that is made up of three structural proteins, the capsid (C), premembrane (prM), and envelope (E) proteins, and seven non-structural (NS) proteins (NS1, NS2A/B, NS3, NS4A, 2K, NS4B, and NS5) (1, 2, 5, 6).

The Flavivirus group antigen antibody binds to the fusion loop at the distal end of domain II (EDII) of protein E. Flavivirus E is a glycoprotein that is the main antigen for membrane fusion between virus and host and entry into the cell, as well as inducing protective immunity by stimulating neutralizing antibodies (2, 4, 6). The E protein is 495 amino acids (aa) in length and has a theoretical molecular weight between 55 - 72 kDa, which varies based on glycosylation sites (2). Flavivirus E protein contains three domains (EDI, EDII, and EDIII) followed by a stem that contents to the helix-transmembrane domains (TMDs, TM1 and TM2) (2, 4, 6). The EDII contains an S-S bridge at its distal end and functions as a fusion loop (FL) for virus-mediated membrane fusion and antibody binding (2). Given the important role of Flavivirus protein E in host cell infection and membrane fusion, it has become a target for flavivirus disease prevention and treatment (2, 5).

Alternative names for Flavivirus group antigen include Envelope Protein and Protein E.

References

1. Ngono, A. E., & Shresta, S. (2018). Immune Response to Dengue and Zika. Annual review of immunology. https://doi.org/10.1146/annurev-immunol-042617-053142

2. Rey, F. A., Stiasny, K., Vaney, M. C., Dellarole, M., & Heinz, F. X. (2018). The bright and the dark side of human antibody responses to flaviviruses: lessons for vaccine design. EMBO reports. https://doi.org/10.15252/embr.201745302

3. Zhang, X., Jia, R., Shen, H., Wang, M., Yin, Z., & Cheng, A. (2017). Structures and Functions of the Envelope Glycoprotein in Flavivirus Infections. Viruses. https://doi.org/10.3390/v9110338

4. Laureti, M., Narayanan, D., Rodriguez-Andres, J., Fazakerley, J. K., & Kedzierski, L. (2018). Flavivirus Receptors: Diversity, Identity, and Cell Entry. Frontiers in immunology. https://doi.org/10.3389/fimmu.2018.02180

5. Boldescu, V., Behnam, M., Vasilakis, N., & Klein, C. D. (2017). Broad-spectrum agents for flaviviral infections: dengue, Zika and beyond. Nature reviews. Drug discovery. https://doi.org/10.1038/nrd.2017.33

6. Crill, W. D., & Chang, G. J. (2004). Localization and characterization of flavivirus envelope glycoprotein cross-reactive epitopes. Journal of virology, 78(24), 13975-13986. https://doi.org/10.1128/JVI.78.24.13975-13986.2004

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Flavivirus group antigen Antibody (NBP2-52709)(11)

We have publications tested in 3 confirmed species: Human, Monkey, Virus.

We have publications tested in 4 applications: ELISA, ICC/IF, IHC, WB.


Filter By Application
ELISA
(3)
ICC/IF
(1)
IHC
(1)
WB
(2)
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Filter By Species
Human
(1)
Monkey
(1)
Virus
(3)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-52709 Applications Species
Wollner C, Richner M, Hassert M, et al. A mRNA-LNP vaccine against Dengue Virus elicits robust, serotype-specific immunity bioRxiv Jan 7 2021 (WB, Human) WB Human
Wollner CJ, Richner M, Hassert MA et al. A Dengue Virus Serotype 1 mRNA-LNP Vaccine Elicits Protective Immune Responses Journal of virology Mar 24 2021 [PMID: 33762420] (WB) WB
Durham N, Agrawal A, Waltari E et al. Functional characterization and lineage analysis of broadly neutralizing human antibodies against dengue virus identified by single B cell transcriptomics bioRxiv 2019 Oct 02 (ELISA, Virus) ELISA Virus
Francese R, Civra A, Donalisio M et al. Anti-Zika virus and anti-Usutu virus activity of human milk and its components PLoS Negl Trop Dis Oct 1 2020 [PMID: 33027261] (IHC, Monkey) IHC Monkey
Riva V, Garbelli A, Brai A et al. Unique Domain for a Unique Target: Selective Inhibitors of Host Cell DDX3X to Fight Emerging Viruses J. Med. Chem. Aug 18 2020 [PMID: 32787106]
Vicenti I, Dragoni F, Giannini A et Al. Development of a Cell-Based Immunodetection Assay for Simultaneous Screening of Antiviral Compounds Inhibiting Zika and Dengue Virus Replication SLAS Discov Mar 18 2020 [PMID: 32186426]
Wang S, Zhang Q, Tiwari SK et al. Wang S, Zhang Q, Tiwari SK et al. Integrin alpha v beta 5 Internalizes Zika Virus during Neural Stem Cells Infection and Provides a Promising Target for Antiviral Therapy. Cell Rep Jan 28 2020 [PMID: 31956073] (ELISA) ELISA
Dragoni F, Boccuto A, Picarazzi F et al. Evaluation of sofosbuvir activity and resistance profile against West Nile virus in vitro Antiviral Res. Jan 10 2020 [PMID: 31931104] (ELISA, Virus) ELISA Virus
Durham ND, Agrawal A, Waltari E et al. Broadly neutralizing human antibodies against dengue virus identified by single B cell transcriptomics Elife Dec 10 2019 [PMID: 31820734] (Virus) Virus
Vogt MB, Lahon A, Arya RP, et al. Dengue viruses infect human megakaryocytes, with probable clinical consequences. PLoS Negl Trop Dis Nov 1 2019 [PMID: 31765380] (ICC/IF) ICC/IF
Show All 11 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Flavivirus group antigen Antibody (NBP2-52709). (Showing 1 - 1 of 1 FAQs).

  1. What is your recommended dilution for this antibody  in Flow Cytometry application?
    • We normally will not have dilutions listed for the conjugated antibodies, only the applications.We would refer you to the unconjugated antibody for the recommended dilution for an application:https://www.novusbio.com/products/flavivirus-group-antigen-antibody-d1-4g2-4-15-4g2-_nbp2-52709

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-52709AF350
Alexa Fluor 488 NBP2-52709AF488
Alexa Fluor 532 NBP2-52709AF532
Alexa Fluor 594 NBP2-52709AF594
Alexa Fluor 647 NBP2-52709AF647
Alexa Fluor 700 NBP2-52709AF700
Alexa Fluor 750 NBP2-52709AF750
Allophycocyanin NBP2-52709APC
Biotin NBP2-52709B
DyLight 350 NBP2-52709UV
DyLight 405 NBP2-52709V
DyLight 488 NBP2-52709G
DyLight 550 NBP2-52709R
DyLight 594 NBP2-52709DL594
DyLight 650 NBP2-52709C
DyLight 680 NBP2-52709FR
DyLight 755 NBP2-52709IR
FITC NBP2-52709F
HRP NBP2-52709H
Janelia Fluor 549 NBP2-52709JF549
Janelia Fluor 646 NBP2-52709JF646
PE NBP2-52709PE
PE/Atto594 NBP2-52709PEATT594
PE/Cy5.5 NBP2-52709PECY55
PE/Cy7 NBP2-52709PECY7
PerCP NBP2-52709PCP

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