Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [NBP2-52709] - Human C3/36 cell line. Image from verified customer review.
Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [NBP2-52709] - Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [PE] [NBP2-52709PE] - Mk2 cell line was infected with ZIKA virus ...read more
ELISA: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [NBP2-52709] - ELISA on Denge Virus-Like Particles using NBP2-52709. Dengue Virus Serotype 2 VLPs were coated onto a plate at 5ug/ml. Anti-flavivirus antibody ...read more
Binding Activity: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [NBP2-52709] - IF assay in a high throughput format for screening Dengue virus inhibitors. HEK 293 cells in DMEM 5% FBS/1% PS at 5000 cells/well ...read more
Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) Summary
Recombinant Monoclonal Antibody
This recombinant Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) was prepared from Dengue Virus type 2 antigens.
This Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) recognises flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.
Protein A purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Dengue Virus, Zika Virus, West Nile Virus, Yellow Fever Virus, Flaviviridae. Monkey reactivity reported in scientific literature (PMID:33027261).
Packaging, Storage & Formulations
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
0.02% Proclin 300
Protein A purified
Alternate Names for Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2))
Flaviviruses are a group of seventy enveloped, positive-sense single stranded RNA viruses belonging to the Flaviviridae family (1-5). Flaviviruses are typically transmitted through arthropods, like mosquitoes and ticks, and can infect humans causing outbreaks and serious illness or death worldwide (1-5). These well-known viral pathogens include dengue virus (DENV), West Nile virus (WNV), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBEV), Yellow Fever virus (YFV), and Zika virus (ZIKV) (1-5). Flaviviruses consist of ~10.7 kb RNA genome with one open reading frame (ORF) that is flanked by 5' and 3' untranslated regions (UTRs) (1, 2, 6). The ORF encodes for a polyprotein that is made up of three structural proteins, the capsid (C), premembrane (prM), and envelope (E) proteins, and seven non-structural (NS) proteins (NS1, NS2A/B, NS3, NS4A, 2K, NS4B, and NS5) (1, 2, 5, 6).
The Flavivirus group antigen antibody binds to the fusion loop at the distal end of domain II (EDII) of protein E. Flavivirus E is a glycoprotein that is the main antigen for membrane fusion between virus and host and entry into the cell, as well as inducing protective immunity by stimulating neutralizing antibodies (2, 4, 6). The E protein is 495 amino acids (aa) in length and has a theoretical molecular weight between 55 - 72 kDa, which varies based on glycosylation sites (2). Flavivirus E protein contains three domains (EDI, EDII, and EDIII) followed by a stem that contents to the helix-transmembrane domains (TMDs, TM1 and TM2) (2, 4, 6). The EDII contains an S-S bridge at its distal end and functions as a fusion loop (FL) for virus-mediated membrane fusion and antibody binding (2). Given the important role of Flavivirus protein E in host cell infection and membrane fusion, it has become a target for flavivirus disease prevention and treatment (2, 5).
Alternative names for Flavivirus group antigen include Envelope Protein and Protein E.
1. Ngono, A. E., & Shresta, S. (2018). Immune Response to Dengue and Zika. Annual review of immunology. https://doi.org/10.1146/annurev-immunol-042617-053142
2. Rey, F. A., Stiasny, K., Vaney, M. C., Dellarole, M., & Heinz, F. X. (2018). The bright and the dark side of human antibody responses to flaviviruses: lessons for vaccine design. EMBO reports. https://doi.org/10.15252/embr.201745302
3. Zhang, X., Jia, R., Shen, H., Wang, M., Yin, Z., & Cheng, A. (2017). Structures and Functions of the Envelope Glycoprotein in Flavivirus Infections. Viruses. https://doi.org/10.3390/v9110338
4. Laureti, M., Narayanan, D., Rodriguez-Andres, J., Fazakerley, J. K., & Kedzierski, L. (2018). Flavivirus Receptors: Diversity, Identity, and Cell Entry. Frontiers in immunology. https://doi.org/10.3389/fimmu.2018.02180
5. Boldescu, V., Behnam, M., Vasilakis, N., & Klein, C. D. (2017). Broad-spectrum agents for flaviviral infections: dengue, Zika and beyond. Nature reviews. Drug discovery. https://doi.org/10.1038/nrd.2017.33
6. Crill, W. D., & Chang, G. J. (2004). Localization and characterization of flavivirus envelope glycoprotein cross-reactive epitopes. Journal of virology, 78(24), 13975-13986. https://doi.org/10.1128/JVI.78.24.13975-13986.2004
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Flavivirus group antigen Antibody (NBP2-52709)(11)
We have publications tested in 3 confirmed species: Human, Monkey, Virus.
We have publications tested in 4 applications: ELISA, ICC/IF, IHC, WB.
Durham N, Agrawal A, Waltari E et al. Functional characterization and lineage analysis of broadly neutralizing human antibodies against dengue virus identified by single B cell transcriptomics bioRxiv 2019 Oct 02 (ELISA, Virus)
FAQs for Flavivirus group antigen Antibody (NBP2-52709). (Showing 1 - 1 of 1 FAQs).
What is your recommended dilution for this antibody in Flow Cytometry application?
We normally will not have dilutions listed for the conjugated antibodies, only the applications.We would refer you to the unconjugated antibody for the recommended dilution for an application:https://www.novusbio.com/products/flavivirus-group-antigen-antibody-d1-4g2-4-15-4g2-_nbp2-52709
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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