Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity Pm, V-ViSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, ICC/IF
Clone
D1-4G2-4-15 (4G2)
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 594

Order Details

Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [Alexa Fluor® 594] Summary

Immunogen
This recombinant Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) was prepared from Dengue Virus type 2 antigens.
Specificity
This Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) recognises flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Dengue Virus, Zika Virus, West Nile Virus, Yellow Fever Virus, Flaviviridae. Monkey reactivity reported in scientific literature (PMID:33027261).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [Alexa Fluor® 594]

  • Envelope Protein
  • Protein E

Background

Flaviviruses are a group of seventy enveloped, positive-sense single stranded RNA viruses belonging to the Flaviviridae family (1-5). Flaviviruses are typically transmitted through arthropods, like mosquitoes and ticks, and can infect humans causing outbreaks and serious illness or death worldwide (1-5). These well-known viral pathogens include dengue virus (DENV), West Nile virus (WNV), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBEV), Yellow Fever virus (YFV), and Zika virus (ZIKV) (1-5). Flaviviruses consist of ~10.7 kb RNA genome with one open reading frame (ORF) that is flanked by 5' and 3' untranslated regions (UTRs) (1, 2, 6). The ORF encodes for a polyprotein that is made up of three structural proteins, the capsid (C), premembrane (prM), and envelope (E) proteins, and seven non-structural (NS) proteins (NS1, NS2A/B, NS3, NS4A, 2K, NS4B, and NS5) (1, 2, 5, 6).

The Flavivirus group antigen antibody binds to the fusion loop at the distal end of domain II (EDII) of protein E. Flavivirus E is a glycoprotein that is the main antigen for membrane fusion between virus and host and entry into the cell, as well as inducing protective immunity by stimulating neutralizing antibodies (2, 4, 6). The E protein is 495 amino acids (aa) in length and has a theoretical molecular weight between 55 - 72 kDa, which varies based on glycosylation sites (2). Flavivirus E protein contains three domains (EDI, EDII, and EDIII) followed by a stem that contents to the helix-transmembrane domains (TMDs, TM1 and TM2) (2, 4, 6). The EDII contains an S-S bridge at its distal end and functions as a fusion loop (FL) for virus-mediated membrane fusion and antibody binding (2). Given the important role of Flavivirus protein E in host cell infection and membrane fusion, it has become a target for flavivirus disease prevention and treatment (2, 5).

Alternative names for Flavivirus group antigen include Envelope Protein and Protein E.

References

1. Ngono, A. E., & Shresta, S. (2018). Immune Response to Dengue and Zika. Annual review of immunology. https://doi.org/10.1146/annurev-immunol-042617-053142

2. Rey, F. A., Stiasny, K., Vaney, M. C., Dellarole, M., & Heinz, F. X. (2018). The bright and the dark side of human antibody responses to flaviviruses: lessons for vaccine design. EMBO reports. https://doi.org/10.15252/embr.201745302

3. Zhang, X., Jia, R., Shen, H., Wang, M., Yin, Z., & Cheng, A. (2017). Structures and Functions of the Envelope Glycoprotein in Flavivirus Infections. Viruses. https://doi.org/10.3390/v9110338

4. Laureti, M., Narayanan, D., Rodriguez-Andres, J., Fazakerley, J. K., & Kedzierski, L. (2018). Flavivirus Receptors: Diversity, Identity, and Cell Entry. Frontiers in immunology. https://doi.org/10.3389/fimmu.2018.02180

5. Boldescu, V., Behnam, M., Vasilakis, N., & Klein, C. D. (2017). Broad-spectrum agents for flaviviral infections: dengue, Zika and beyond. Nature reviews. Drug discovery. https://doi.org/10.1038/nrd.2017.33

6. Crill, W. D., & Chang, G. J. (2004). Localization and characterization of flavivirus envelope glycoprotein cross-reactive epitopes. Journal of virology, 78(24), 13975-13986. https://doi.org/10.1128/JVI.78.24.13975-13986.2004

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Flavivirus group antigen Antibody (NBP2-52709AF594). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in ordering the Flavivirus group antigen [D1-4G2-4-15 (4G2)] monoclonal antibody with the Mouse IgG2a isotype (catalog number NBP2-52709). We are aware that the  hybridoma D1-4G2-4-15 (4G2)  produces both IgG2 and IgG1 antibodies. Because of the isotype specifictiy of the application, we would like to confirm that the isotype is purely IgG2a, as stated on the product information page, and not a mix.
    • The two different hybridoma products differ by the host and catalog numbers (NBP2-52709, NBP2-52666), and are not mixed. So if you are looking to get a Mouse IgG2A to your secondary, you can please take a look at NBP2-52709 and see if it is suitable for you.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-52709AF350
Alexa Fluor 488 NBP2-52709AF488
Alexa Fluor 532 NBP2-52709AF532
Alexa Fluor 594 NBP2-52709AF594
Alexa Fluor 647 NBP2-52709AF647
Alexa Fluor 700 NBP2-52709AF700
Alexa Fluor 750 NBP2-52709AF750
Allophycocyanin NBP2-52709APC
Biotin NBP2-52709B
DyLight 350 NBP2-52709UV
DyLight 405 NBP2-52709V
DyLight 488 NBP2-52709G
DyLight 550 NBP2-52709R
DyLight 594 NBP2-52709DL594
DyLight 650 NBP2-52709C
DyLight 680 NBP2-52709FR
DyLight 755 NBP2-52709IR
FITC NBP2-52709F
HRP NBP2-52709H
Janelia Fluor 549 NBP2-52709JF549
Janelia Fluor 646 NBP2-52709JF646
PE NBP2-52709PE
PE/Atto594 NBP2-52709PEATT594
PE/Cy5.5 NBP2-52709PECY55
PE/Cy7 NBP2-52709PECY7
PerCP NBP2-52709PCP

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