| Reactivity | BaSpecies Glossary |
| Applications | ELISA |
| Clone | 121 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Janelia Fluor 549 |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was obtained from a rabbit immunized with purified, recombinant Listeria monocytogenes Flagellin (YP_013330.1; Met1-Ser287). |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-90335AF350 | |
| Alexa Fluor 405 | NBP2-90335AF405 | |
| Alexa Fluor 488 | NBP2-90335AF488 | |
| Alexa Fluor 532 | NBP2-90335AF532 | |
| Alexa Fluor 594 | NBP2-90335AF594 | |
| Alexa Fluor 647 | NBP2-90335AF647 | |
| Alexa Fluor 700 | NBP2-90335AF700 | |
| Alexa Fluor 750 | NBP2-90335AF750 | |
| Biotin | NBP2-90335B | |
| DyLight 350 | NBP2-90335UV | |
| DyLight 405 | NBP2-90335V | |
| DyLight 488 | NBP2-90335G | |
| DyLight 550 | NBP2-90335R | |
| DyLight 594 | NBP2-90335DL594 | |
| DyLight 650 | NBP2-90335C | |
| DyLight 680 | NBP2-90335FR | |
| DyLight 755 | NBP2-90335IR | |
| FITC | NBP2-90335F | |
| HRP | NBP2-90335H | |
| Janelia Fluor 549 | NBP2-90335JF549 | |
| Janelia Fluor 646 | NBP2-90335JF646 |
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")