![Western Blot: Flagellin Antibody (06) [NBP3-05967] - Listeria monocytogenes flagellin / flaA Recombinant Protein Lane A: 10ng. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.](http://images.novusbio.com/fullsize/Flagellin-Antibody-06-Western-Blot-NBP3-05967-img0002.jpg "Western Blot: Flagellin Antibody (06) [NBP3-05967] - Listeria monocytogenes flagellin / flaA Recombinant Protein Lane A: 10ng. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.")
| Reactivity | BaSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 06 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Listeria monocytogenes Flagellin (Accession#: YP_013330.1; Met1-Ser287). |
| Specificity | No cross-reactivity in ELISA with: E. coli cell lysate |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Flagellin Antibody (06) [NBP3-05967] - Listeria monocytogenes flagellin / flaA Recombinant Protein Lane A: 200ngLane B: 50ng. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.](http://images.novusbio.com/fullsize/Flagellin-Antibody-06-Western-Blot-NBP3-05967-img0001.jpg "Western Blot: Flagellin Antibody (06) [NBP3-05967] - Listeria monocytogenes flagellin / flaA Recombinant Protein Lane A: 200ngLane B: 50ng. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")