![Western Blot: FIV Matrix protein p15 Antibody [NBP3-11648] - Western blot using affinity purified anti-FIV Matrix Protein p15 to detect p15 in the culture supernatant of FIV-infected feline CrFK cells (lane 2, arrowhead). Lane 1 is an uninfected control. Virions were enriched by ultracentrifugation, lysed, resolved by electrophoresis, and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:10,000 dilution.](http://images.novusbio.com/fullsize/FIV-Matrix-protein-p15-Antibody-Western-Blot-NBP3-11648-img0001.jpg "Western Blot: FIV Matrix protein p15 Antibody [NBP3-11648] - Western blot using affinity purified anti-FIV Matrix Protein p15 to detect p15 in the culture supernatant of FIV-infected feline CrFK cells (lane 2, arrowhead). Lane 1 is an uninfected control. Virions were enriched by ultracentrifugation, lysed, resolved by electrophoresis, and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:10,000 dilution.")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | This affinity purified antibody is directed against FIV Matrix Protein p15. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of FIV Matrix Protein p15 (Uniprot: P16087). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | gag |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This affinity purified antibody has been tested for use in ELISA and western blotting and suitable for immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15 kDa in size corresponding to FIV Matrix Protein p15 by western blotting in the appropriate cell lysate or extract. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
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