FITC Antibody - Azide and BSA Free

Fluorophore-linked immunosorbent assay: FITC Antibody [NB100-64617] - FLISA plate was coated with Goat Anti-Human IgG-FITC and Goat Anti-Human IgG-BIOT. Antibodies were detected with serially diluted Sheep Anti-FITC-PE. Image using the Alkaline Phosphatase form of this antibody.

• Reactivity: All-NA
• Applications: WB, ELISA, Flow
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

FITC Antibody - Azide and BSA Free Summary

• Immunogen: Pooled antisera from sheep hyperimmunized with fluorescein isothiocyanate (Isomer I)
• Specificity: Recognizes Fluorescein isothiocyanate. Cross Adsorption None; may react with other fluorescein derivatives
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Fluorophore-linked immunosorbent assay
  • Western Blot 1:100-1:2000
• Application Notes: WB reactivity reported in (PMID: 24166503).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FITC Antibody - Azide and BSA Free

• FITC
• Fluorescein Isothiocyanate

Background

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FITC Antibody (NB100-64617)(2)

Publications using NB100-64617

• Fujiya M, Konishi H, Mohamed Kamel MK et al. microRNA-18a induces apoptosis in colon cancer cells via the autophagolysosomal degradation of oncogenic heterogeneous nuclear ribonucleoprotein A1 Oncogene 2013-10-28 [PMID: 24166503] (WB)
  
  Details:
  This citation used the Alkaline Phosphatase version of this antibody.
• Liao WC, Chuang MC, Ho JA et al. Electrochemical Sensor for Multiplex Screening of Genetically Modified DNA: Identification of Biotech Crops by Logic-Based Biomolecular Analysis Biosens Bioelectron 2013-07-13 [PMID: 23893064]
  
  Details:
  This citation used the HRP version of this antibody.