FITC Antibody

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Flow Cytometry: FITC Antibody [NB100-64617] - FLISA plate was coated with Goat Anti-Human IgG-FITC and Goat Anti-Human IgG-BIOT. Antibodies were detected with serially diluted Sheep Anti-FITC-PE. Image using the ...read more

Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, ELISA, Flow
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates

FITC Antibody Summary

Immunogen
Pooled antisera from sheep hyperimmunized with fluorescein isothiocyanate (Isomer I)
Specificity
Recognizes Fluorescein isothiocyanate. Cross Adsorption None; may react with other fluorescein derivatives
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
Publications
Read Publications using NB100-64617.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FITC Antibody

  • FITC
  • Fluorescein Isothiocyanate

Background

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FITC Antibody (NB100-64617)(4)

We have publications tested in 1 application: WB.


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WB
(2)
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Showing Publications 1 - 4 of 4.
Publications using NB100-64617 Applications Species
Fujiya M, Konishi H, Mohamed Kamel MK et al. microRNA-18a induces apoptosis in colon cancer cells via the autophagolysosomal degradation of oncogenic heterogeneous nuclear ribonucleoprotein A1 Oncogene (WB)

Details:
This publication used the Alkaline Phosphatase conjugated form of this antibody (Cat# NB100-64618).
WB
Fujiya M, Konishi H, Mohamed Kamel MK et al. microRNA-18a induces apoptosis in colon cancer cells via the autophagolysosomal degradation of oncogenic heterogeneous nuclear ribonucleoprotein A1 Oncogene 2013 Oct 28 [PMID: 24166503] (WB)

Details:
This citation used the Alkaline Phosphatase version of this antibody.
WB
Liao WC, Chuang MC, Ho JA et al. Electrochemical Sensor for Multiplex Screening of Genetically Modified DNA: Identification of Biotech Crops by Logic-Based Biomolecular Analysis Biosens Bioelectron 2013 Jul 13 [PMID: 23893064]

Details:
This citation used the HRP version of this antibody.
Liao WC, Chuang MC, Ho JA et al. Electrochemical Sensor for Multiplex Screening of Genetically Modified DNA: Identification of Biotech Crops by Logic-Based Biomolecular Analysis Biosens Bioelectron

Details:
This publication used the HRP conjugated form of this antibody (Cat# NB100-62570).

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Product General Protocols

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Video Protocols

WB Video Protocol

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