FITC Antibody - Azide and BSA Free

Images

 
Fluorophore-linked immunosorbent assay: FITC Antibody [NB100-64617] - FLISA plate was coated with Goat Anti-Human IgG-FITC and Goat Anti-Human IgG-BIOT. Antibodies were detected with serially diluted Sheep Anti-FITC-PE. ...read more

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, ELISA, FLISA, Flow
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

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FITC Antibody - Azide and BSA Free Summary

Immunogen
Pooled antisera from sheep hyperimmunized with fluorescein isothiocyanate (Isomer I)
Specificity
Recognizes Fluorescein isothiocyanate. Cross Adsorption None; may react with other fluorescein derivatives
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Fluorophore-linked immunosorbent assay
  • Western Blot 1:100-1:2000
Application Notes
WB reactivity reported in (PMID: 24166503).
Publications
Read Publications using NB100-64617.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FITC Antibody - Azide and BSA Free

  • FITC
  • Fluorescein Isothiocyanate

Background

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FITC Antibody (NB100-64617)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Array NB100-64617

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