FITC Antibody [Alkaline Phosphatase]

Fluorophore-linked immunosorbent assay: FITC Antibody [Alkaline Phosphatase] [NB100-64618] - FLISA plate was coated with Goat Anti-Human IgG-FITC and Goat Anti-Human IgG-BIOT. Antibodies were detected with serially diluted Sheep Anti-FITC-PE.

• Reactivity: All-NA
• Applications: WB, ELISA, Flow
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alkaline Phosphatase

FITC Antibody [Alkaline Phosphatase] Summary

• Immunogen: Pooled antisera from sheep hyperimmunized with fluorescein isothiocyanate (Isomer I)
• Specificity: Recognizes Fluorescein isothiocyanate. Cross Adsorption None; may react with other fluorescein derivatives
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000-1:4000
  • Flow Cytometry
  • Fluorophore-linked immunosorbent assay 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: WB reactivity reported in (PMID: 24166503).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris (pH 8.0), 1 mM MgCl2, and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for FITC Antibody [Alkaline Phosphatase]

• FITC
• Fluorescein Isothiocyanate

Background

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FITC Antibody (NB100-64618)(1)

Publication using NB100-64618

• Fujiya M, Konishi H, Mohamed Kamel MK et al. microRNA-18a induces apoptosis in colon cancer cells via the autophagolysosomal degradation of oncogenic heterogeneous nuclear ribonucleoprotein A1 Oncogene 2013-10-28 [PMID: 24166503] (WB)
  
  Details:
  This citation used the Alkaline Phosphatase version of this antibody.

FAQs for FITC Antibody (NB100-64618). (Showing 1 - 1 of 1 FAQ).

1. How many alkaline phosphatases are bound to one IgG? And I want to know the labeling method. What is the IgG site crosslinking alkaline phosphatase? NH2 or SH site?
   • We do not measure the amount of AP bound to the immunoglobulins. We conjugate the antibody with an over-saturation of AP which may result in free AP in the product. The AP binds to cysteine.