FITC Antibody [Alkaline Phosphatase]

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Fluorophore-linked immunosorbent assay: FITC Antibody [Alkaline Phosphatase] [NB100-64618] - FLISA plate was coated with Goat Anti-Human IgG-FITC and Goat Anti-Human IgG-BIOT. Antibodies were detected with serially ...read more

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, ELISA, FLISA, Flow
Clonality
Polyclonal
Host
Sheep
Conjugate
Alkaline Phosphatase

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Catalog# & Conjugate Size Price

FITC Antibody [Alkaline Phosphatase] Summary

Immunogen
Pooled antisera from sheep hyperimmunized with fluorescein isothiocyanate (Isomer I)
Specificity
Recognizes Fluorescein isothiocyanate. Cross Adsorption None; may react with other fluorescein derivatives
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:2000-1:4000
  • Flow Cytometry
  • Fluorophore-linked immunosorbent assay 1:100-1:2000
Application Notes
WB reactivity reported in (PMID: 24166503).
Publications
Read Publication using NB100-64618.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50 mM Tris (pH 8.0), 1 mM MgCl2, and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FITC Antibody [Alkaline Phosphatase]

  • FITC
  • Fluorescein Isothiocyanate

Background

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FITC Antibody (NB100-64618)(1)

We have publications tested in 1 application: WB.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FITC Antibody (NB100-64618). (Showing 1 - 1 of 1 FAQ).

  1. How many alkaline phosphatases are bound to one IgG? And I want to know the labeling method. What is the IgG site crosslinking alkaline phosphatase? NH2 or SH site?
    • We do not measure the amount of AP bound to the immunoglobulins. We conjugate the antibody with an over-saturation of AP which may result in free AP in the product. The AP binds to cysteine.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alkaline Phosphatase NB100-64618
Biotin NB100-64619
HRP NB100-62570

Additional FITC Products

Array NB100-64618

Blogs on FITC

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