FITC Antibody [HRP]

Images

 
ELISA: FITC Antibody [HRP] [NB100-62570] - FLISA plate was coated with Goat Anti-Human IgG-FITC and Goat Anti-Human IgG-BIOT. Antibodies were detected with serially diluted Sheep Anti-FITC-PE

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications ELISA, Flow
Clonality
Polyclonal
Host
Sheep
Conjugate
HRP

Order Details

View Available Conjugates

FITC Antibody [HRP] Summary

Immunogen
Pooled antisera from sheep hyperimmunized with fluorescein isothiocyanate (Isomer I)
Specificity
Recognizes Fluorescein isothiocyanate. Cross Adsorption None; may react with other fluorescein derivatives
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:2000 - 1:4000
  • Flow Cytometry 0.1 ug/mL
Readout System
Publications
Read Publication using NB100-62570.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50% Glycerol/50% PBS (pH 7.4)
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for FITC Antibody [HRP]

  • FITC
  • Fluorescein Isothiocyanate

Background

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FITC Antibody (NB100-62570)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FITC Antibody (NB100-62570). (Showing 1 - 2 of 2 FAQ).

  1. Upon the customer's inquiry, may we ask the concentration of NB100-62570? The lot no. is G6306-S411B.
    • I found out that for your lot# the concentration is 0.35 mg/mL.
  2. I have a question about FITC Antibody [HRP](NB100-62570). Please tell me what is the molecular weight? Also please tell me how energy HRP is bonded with FITC antibody?
    • FITC is a small molecule that will be less than 1 kDa on Western blot. The molecular weight you will observe will depend on the weight of the protein that FITC is attached to. The HRP is covalently bonded to the antibody via the free amine groups on the antibody.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alkaline Phosphatase NB100-64618
Biotin NB100-64619
HRP NB100-62570

Additional FITC Products

Array NB100-62570

Blogs on FITC

There are no specific blogs for FITC, but you can read our latest blog posts.

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Calculators

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