FITC Antibody [HRP]

ELISA: FITC Antibody [HRP] [NB100-62570] - FLISA plate was coated with Goat Anti-Human IgG-FITC and Goat Anti-Human IgG-BIOT. Antibodies were detected with serially diluted Sheep Anti-FITC-PE

• Reactivity: All-NA
• Applications: ELISA, Flow
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: HRP

FITC Antibody [HRP] Summary

• Immunogen: Pooled antisera from sheep hyperimmunized with fluorescein isothiocyanate (Isomer I)
• Specificity: Recognizes Fluorescein isothiocyanate. Cross Adsorption None; may react with other fluorescein derivatives
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000 - 1:4000
  • Flow Cytometry 0.1 ug/mL
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/50% PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for FITC Antibody [HRP]

• FITC
• Fluorescein Isothiocyanate

Background

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FITC Antibody (NB100-62570)(1)

Publication using NB100-62570

• Liao WC, Chuang MC, Ho JA et al. Electrochemical Sensor for Multiplex Screening of Genetically Modified DNA: Identification of Biotech Crops by Logic-Based Biomolecular Analysis Biosens Bioelectron 2013-07-13 [PMID: 23893064]
  
  Details:
  This citation used the HRP version of this antibody.

FAQs for FITC Antibody (NB100-62570). (Showing 1 - 2 of 2 FAQ).

1. Upon the customer's inquiry, may we ask the concentration of NB100-62570? The lot no. is G6306-S411B.
   • I found out that for your lot# the concentration is 0.35 mg/mL.
2. I have a question about FITC Antibody [HRP](NB100-62570). Please tell me what is the molecular weight? Also please tell me how energy HRP is bonded with FITC antibody?
   • FITC is a small molecule that will be less than 1 kDa on Western blot. The molecular weight you will observe will depend on the weight of the protein that FITC is attached to. The HRP is covalently bonded to the antibody via the free amine groups on the antibody.